人DMPK (Dystrophia myotonica蛋白kinase) knockout HEK-293T cell裂解物
Human DMPK (Dystrophia myotonica protein kinase) knockout HEK-293T cell lysate
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DMPK KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
查看别名
DM, DM 1, DM protein kinase, DM-kinase, DM1 protein kinase, DM1PK, DMK, DMPK_HUMAN, Dystrophia myotonica 1, Dystrophia myotonica protein kinase, MDPK, MT-PK, Myotonic dystrophy associated protein kinase, Myotonic dystrophy protein kinase, Myotonin protein kinase A, Myotonin-protein kinase, Thymopoietin homolog
- Sanger seq
Unknown
Sanger Sequencing - Human DMPK (Dystrophia myotonica protein kinase) knockout HEK-293T cell lysate (AB258844)
Homozygous : 1 bp insertion in exon1
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DMPK acts to regulate muscle function and integrity by modulating phosphorylation states within cells. It is not typically part of known multi-protein complexes but works alongside other kinases to affect signaling pathways. For example DMPK can influence actin filaments and myosin phosphorylation contributing to muscle cell contractility and stability. This regulatory function is essential for the maintenance of normal muscle tone and function.
Pathways
DMPK participates actively in RhoA and MAPK signaling pathways. DMPK impacts the RhoA pathway by regulating cytoskeletal organization and cell morphology interacting with proteins like Rho kinase to modulate muscle contraction. In the MAPK pathway DMPK plays a role in stress response signaling impacting proteins such as ERK that control gene expression in response to stressors.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
搭配使用产品
Cell Lines & Lysates
AB266870
Human DMPK (Dystrophia myotonica protein kinase) knockout HCT116 cell line
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Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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