AB257406

人CYR61 (CCN1) knockout HeLa cell裂解物

Name: 人CYR61 (CCN1) knockout HeLa cell lysate (ab257406)| Abcam中文官网
Brand: Abcam Limited
SKU: AB257406
Price: 6206 CNY
Availability: InStock

Human CYR61 (CCN1) knockout HeLa cell lysate

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CCN1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.

查看别名

3CH61, CCN family member 1, CYR 61, CYR61 protein, CYR61_HUMAN, Cysteine rich heparin binding protein 61, Cysteine-rich angiogenic inducer 61, GIG 1, IBP-10, IGF-binding protein 10, IGFBP-10, Insulin-like growth factor-binding protein 10, Protein CYR61, Protein GIG1

3 Images

Lab

Western blot - Human CYR61 (CCN1) knockout HeLa cell lysate (AB257406)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : CYR61 knockout HeLa cell lysate (20 ug)
Lane 3 : MDA-MB-231 cell lysate (20 ug)
Lane 4 : MCF7 cell lysate (20 ug)

ab230947 was shown to specifically react with CYR61/CCN1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265288 (knockout cell lysate ab257406) was used. Wild-type and CYR61/CCN1 knockout samples were subjected to SDS-PAGE. ab230947 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CYR61/CCN1 antibody [EPR20681] (<a href='/products/primary-antibodies/cyr61-ccn1-antibody-epr20681-ab230947'>ab230947</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

CYR61 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human CYR61 (CCN1) knockout HeLa cell line (<a href='/products/cell-lines/human-cyr61-ccn1-knockout-hela-cell-line-ab265288'>ab265288</a>)

Lane 3:

MDA-MB-231 cell lysate at 20 µg

Lane 4:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 47 kDa

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Sanger Sequencing - Human CYR61 (CCN1) knockout HeLa cell lysate (AB257406)

Sanger seq

Unknown

Sanger Sequencing - Human CYR61 (CCN1) knockout HeLa cell lysate (AB257406)

Allele-1 : 1 bp deletion in exon1

Sanger seq

Unknown

Sanger Sequencing - Human CYR61 (CCN1) knockout HeLa cell lysate (AB257406)

Allele-2 : Insertion of the selection cassette in exon1

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.

疾病

Adenocarcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称

CCN1

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The CYR61/CCN1 protein also known simply as CYR61 CCN1 or CYR61 protein is a cellular protein with a molecular weight of approximately 40 kDa. It is a member of the CCN family of regulatory proteins which includes connective tissue growth factors. CYR61 is secreted into the extracellular matrix and has a critical role in cell adhesion and signaling. The protein is expressed in various tissues with higher levels observed in the placenta lungs and kidneys reflecting its role in organ development and repair processes.

Biological function summary

The CYR61/CCN1 protein acts as a signaling molecule that influences cell proliferation migration and adhesion. It interacts with integrins and heparan sulfate proteoglycans on the cell surface facilitating cellular communication that is essential for structural and functional tissue integrity. Additionally CYR61 is a part of multiprotein complexes impacting angiogenesis and inflammation. These activities place CYR61/CCN1 at a pivotal point in wound healing and vascularization processes.

Pathways

The CYR61/CCN1 protein participates in key signaling pathways such as the MAPK/ERK and Wnt pathways. These pathways modulate gene expression related to cell growth and differentiation. CYR61 collaborates with other proteins including VEGF and FGF to regulate cardiovascular development and morphogenesis. The interaction with these pathways highlights the protein’s integral role in cellular homeostasis and response to physiological changes.

CYR61/CCN1 is linked to cancer progression and fibrotic diseases. Elevated levels of this protein are frequently found in breast cancer emphasizing its role in tumor growth and metastasis. It associates with other proteins like TGF-beta during fibrotic responses contributing to the pathology of fibrosis in organs such as the liver and lungs. The involvement in these processes highlights its potential as a therapeutic target in oncology and fibrotic disease management.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

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人CYR61 (CCN1) knockout HeLa cell裂解物

Human CYR61 (CCN1) knockout HeLa cell lysate

Western blot - Human CYR61 (CCN1) knockout HeLa cell lysate (AB257406)

Sanger Sequencing - Human CYR61 (CCN1) knockout HeLa cell lysate (AB257406)

Sanger Sequencing - Human CYR61 (CCN1) knockout HeLa cell lysate (AB257406)