人CYP24A1 knockout A549 cell裂解物(ab273777)
概述
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产品名称
人CYP24A1 knockout A549 cell裂解物 -
产品概述
Knockout cell lysate achieved by CRISPR/Cas9.
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Parental Cell Line
A549 -
Organism
Human -
Mutation description
Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift: 98% -
Passage number
<20 -
Knockout validation
Next Generation Sequencing (NGS) -
Reconstitution notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT. *Usage of SDS sample buffer is not recommended with these lyophilized lysates. -
说明
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
See here for more information on knockout cell lysates.Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
性能
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存放说明
Store at -80°C. Please refer to protocols. -
组件 1 kit ab280627 - Human CYP24A1 knockout A549 cell lysate 1 x 100µg ab259782 - Human wild-type A549 cell lysate 1 x 100µg -
研究领域
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Cell type
epithelial -
Disease
Carcinoma -
Gender
Male
靶标
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功能
Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. -
序列相似性
Belongs to the cytochrome P450 family. -
细胞定位
Mitochondrion. - Information by UniProt
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别名
- 1
- 1 25 @dihydroxyvitamin D3 24 hydroxylase
- 1 25 dihydroxyvitamin D(3) 24 hydroxylase
see all
相关产品
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KO cell lines
图片
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Next Generation Sequencing - Human CYP24A1 knockout A549 cell lysate (ab273777)Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift: 98%
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab273777 尚未被引用在任何文献中。