人CSNK1D (Casein Kinase 1 delta) knockout HeLa cell裂解物
Human CSNK1D (Casein Kinase 1 delta) knockout HeLa cell lysate
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CSNK1D KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp insertion in exon 1 and 1 bp deletion in exon 1.
查看别名
CKI-delta, CKId, CSNK 1D, Casein Kinase 1 delta, Casein kinase I delta isoform, Casein kinase I isoform delta, HCKID, KC1D_HUMAN, Protein kinase CK1 delta
- WB
Unknown
Western blot - Human CSNK1D (Casein Kinase 1 delta) knockout HeLa cell lysate (AB258383)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : CSNK1D knockout HeLa cell lysate (20 μg)
Lane 3 : K-562 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab85320 observed at 47 kDa. Red - loading control, ab181602 observed at 37 kDa.
ab85320 Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] was shown to specifically react with Casein Kinase 1 delta/CSNK1D in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264667 (knockout cell lysate ab258383) was used. Wild-type and Casein Kinase 1 delta/CSNK1D knockout samples were subjected to SDS-PAGE. ab85320 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] (<a href='/products/primary-antibodies/casein-kinase-1-delta-csnk1d-antibody-af12g4-ab85320'>ab85320</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
CSNK1D knockout HeLa cell lysate at 20 µg
Lane 3:
K-562 cell lysate at 20 µg
Predicted band size: 47 kDa
Observed band size: 47 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human CSNK1D (Casein Kinase 1 delta) knockout HeLa cell lysate (AB258383)
Allele-2 : 11 bp insertion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human CSNK1D (Casein Kinase 1 delta) knockout HeLa cell lysate (AB258383)
Allele-1 : 1 bp deletion in exon 1
反应性数据
产品详情
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
搭配使用产品
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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