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AB261683

人CHMP2B knockout A549 cell裂解物

Human CHMP2B knockout A549 cell lysate

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CHMP2B KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%.

查看别名

ALS17, CHM2B_HUMAN, CHMP family, member 2B, CHMP2.5, Charged multivesicular body protein 2b, Chromatin-modifying protein 2b, VPS2 homolog B, VPS2B, Vacuolar protein sorting 2, yeast, homolog of, B, Vacuolar protein sorting 2-2, Vacuolar protein sorting-associated protein 2-2, Vps2-2, hVps2-2

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Western blot - Human CHMP2B knockout A549 cell lysate (AB261683)
  • WB

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Western blot - Human CHMP2B knockout A549 cell lysate (AB261683)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : CHMP2B knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate 20 ug

Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab33174 observed at 30 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab33174 was shown to recognize CHMP2B in wild-type A549 cells as signal was lost at the expected MW in CHMP2B knockout cell line ab261874 (knockout cell lysate ab261683). Additional cross-reactive bands were observed in the wild-type and knockout samples. Wild-type and CHMP2B knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab33174 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CHMP2B antibody (<a href='/products/primary-antibodies/chmp2b-antibody-ab33174'>ab33174</a>) at 1 µg/mL

Lane 1:

Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

CHMP2B knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CHMP2B knockout A549 cell line (<a href='/products/cell-lines/human-chmp2b-knockout-a549-cell-line-ab261874'>ab261874</a>)

Lane 3:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 24 kDa

false

Western blot - Human CHMP2B knockout A549 cell lysate (AB261683)
  • WB

Lab

Western blot - Human CHMP2B knockout A549 cell lysate (AB261683)

Lane 1 : Wild-type A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 2 : CHMP2B knockout A549 (Human lung carcinoma cell line) whole cell lysate 20 ug

Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate 20 ug

Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate 20 ug

Lanes 1 - 4 : Merged signal (red and green). Green - ab157208 observed at 45 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab157208 was shown to specifically react with CHMP2B in wild-type A549 cells as signal was lost in CHMP2B knockout cell line ab261874 (knockout cell lysate ab261683). Wild-type and CHMP2B knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. ab157208 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/1000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CHMP2B antibody [EPR10807(B)] (<a href='/products/primary-antibodies/chmp2b-antibody-epr10807b-ab157208'>ab157208</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 whole cell lysate at 20 µg

Lane 2:

CHMP2B knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human CHMP2B knockout A549 cell line (<a href='/products/cell-lines/human-chmp2b-knockout-a549-cell-line-ab261874'>ab261874</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Predicted band size: 24 kDa

false

Next Generation Sequencing - Human CHMP2B knockout A549 cell lysate (AB261683)
  • NGS

Lab

Next Generation Sequencing - Human CHMP2B knockout A549 cell lysate (AB261683)

X = 1 bp insertion

关键信息

细胞类型

A549

种属

Human

组织

Lung

敲除验证

Next Generation Sequencing,Western blot

突变描述

Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99%

疾病

Carcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
CHMP2B
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Next Generation Sequencing, Western blot
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 1 US: 1

贴壁/悬浮

Adherent

性别

Male

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产品实验方案

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搭配使用产品

Primary Antibodies

AB33174

Anti-CHMP2B antibody

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-CHMP2B antibody (AB33174)

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Proteins & Peptides

AB109959

Recombinant Human CHMP2B protein

SDS-PAGE - Recombinant Human CHMP2B protein (AB109959)

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  • 0

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详情请参阅我们的条款与条件。

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