AB257370

人BAZ1B (WSTF) knockout HeLa cell裂解物

Name: 人BAZ1B (WSTF) knockout HeLa cell lysate (ab257370)| Abcam中文官网
Brand: Abcam Limited
SKU: AB257370
Price: 6206 CNY
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Human BAZ1B (WSTF) knockout HeLa cell lysate

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BAZ1B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.

查看别名

BAZ1B_HUMAN, Bromodomain adjacent to zinc finger domain protein 1B, Tyrosine-protein kinase BAZ1B, WALP-2, WBRS-9, WBSC-10, WBSCR10, WBSCR9, Williams Beuren syndrome chromosome region 9 protein, Williams syndrome transcription factor, Williams-Beuren syndrome chromosomal region 10 protein, Williams-Beuren syndrome chromosomal region 9 protein, hWALP-2, transcription factor WSTF

3 Images

Lab

Western blot - Human BAZ1B (WSTF) knockout HeLa cell lysate (AB257370)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : BAZ1B knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab109439 observed at 171 kDa. Red - loading control ab7291 observed at 50 kDa.
ab109439 Anti-WSTF antibody [EPR1703] was shown to specifically react with WSTF in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264907 (knockout cell lysate ab257370) was used. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109439 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4^°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-WSTF antibody [EPR1703] (<a href='/products/primary-antibodies/wstf-antibody-epr1703-ab109439'>ab109439</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

WSTF knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human BAZ1B (WSTF) knockout HeLa cell line (<a href='/products/cell-lines/human-baz1b-wstf-knockout-hela-cell-line-ab264907'>ab264907</a>)

Predicted band size: 171 kDa

Observed band size: 171 kDa

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Lab

Western blot - Human BAZ1B (WSTF) knockout HeLa cell lysate (AB257370)

Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : BAZ1B knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab51256 observed at 175 kDa. Red - loading control ab7291 observed at 50 kDa.
ab51256 Anti-WSTF antibody [EP1704Y] was shown to specifically react with WSTF in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab264907 (knockout cell lysate ab257370) was used. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab51256 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4^°C at 1 in 15000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-WSTF antibody [EP1704Y] (<a href='/products/primary-antibodies/wstf-antibody-ep1704y-ab51256'>ab51256</a>) at 1/15000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

BAZ1B knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human BAZ1B (WSTF) knockout HeLa cell line (<a href='/products/cell-lines/human-baz1b-wstf-knockout-hela-cell-line-ab264907'>ab264907</a>)

Predicted band size: 171 kDa

Observed band size: 175 kDa

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Sanger Sequencing - Human BAZ1B (WSTF) knockout HeLa cell lysate (AB257370)

Sanger seq

Unknown

Sanger Sequencing - Human BAZ1B (WSTF) knockout HeLa cell lysate (AB257370)

Homozygous : 5 bp deletion in exon 2

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.

疾病

Adenocarcinoma

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反应性数据

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称

BAZ1B

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

合子性

Homozygous

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

WSTF also known as Williams Syndrome Transcription Factor or BAZ1B is a versatile protein with an estimated mass around 175 kDa. It is a component of the nucleosome remodeling factor (NURF) and the Williams-Beuren syndrome chromosome region 17 (WBSCR17) complex. WSTF has widespread expression in various tissues including the brain and heart which highlights its involvement in diverse cellular processes. Functionally it facilitates chromatin remodeling by altering the structure of nucleosomes which is vital for DNA accessibility in transcription DNA repair and replication.

Biological function summary

Since WSTF participates in chromatin structure modulation it plays a considerable role in transcriptional regulation. It is a part of the NURF complex which makes it essential for DNA accessibility and gene expression. The complex interacts with other proteins to allow chromatin to accommodate active transcription by repositioning nucleosomes. WSTF also has a kinase domain that phosphorylates histone H2A. This phosphorylation integrates signals that coordinate transcription and DNA damage repair mechanisms highlighting its multifunctional nature in maintaining genomic stability.

Pathways

WSTF has pivotal roles in the chromatin remodeling and DNA repair pathways. The chromatin remodeling pathway involves WSTF's interaction with the transcription factor complex influencing gene accessibility and expression. WSTF in the DNA repair pathway ensures proper genomic integrity through its association with proteins like BRCA1 highlighting its participation in the cellular response to DNA damage. By engaging with these pathways WSTF contributes to the cell's ability to regulate the genome and respond to damage efficiently.

WSTF is significantly connected to Williams-Beuren syndrome and certain cancers. Williams-Beuren syndrome a developmental disorder arises from the deletion of the region on chromosome 7 involving the WSTF gene affecting brain and heart function. In certain cancers aberrant expression or mutations within the WSTF gene are associated with uncontrolled chromatin remodeling contributing to tumorigenesis. Through these disorders WSTF shows its interaction with proteins like SMARCA1 in chromatin remodeling anomalies emphasizing its importance in disease progression and cellular dysfunction.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

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人BAZ1B (WSTF) knockout HeLa cell裂解物

Human BAZ1B (WSTF) knockout HeLa cell lysate

Western blot - Human BAZ1B (WSTF) knockout HeLa cell lysate (AB257370)

Western blot - Human BAZ1B (WSTF) knockout HeLa cell lysate (AB257370)

Sanger Sequencing - Human BAZ1B (WSTF) knockout HeLa cell lysate (AB257370)