AB257814

人ADAM9 knockout HeLa cell裂解物

Name: 人ADAM9 knockout HeLa cell lysate (ab257814)| Abcam中文官网
Brand: Abcam Limited
SKU: AB257814
Price: 6206 CNY
Availability: InStock

Human ADAM9 knockout HeLa cell lysate

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ADAM9 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

查看别名

A disintegrin and metalloproteinase domain 9, ADAM metallopeptidase domain 9, CORD9, Cellular disintegrin-related protein, Cone rod dystrophy 9, Disintegrin and metalloproteinase domain 9, Disintegrin and metalloproteinase domain-containing protein 9, KIAA0021, MCMP, MDC 9, Meltrin-gamma, Metalloprotease disintegrin cysteine rich protein 9, Mltng, Myeloma cell metalloproteinase

2 Images

Lab

Western blot - Human ADAM9 knockout HeLa cell lysate (AB257814)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : ADAM9 knockout HeLa cell lysate (20 μg)

Lane 3 : HepG2 cell lysate (20 μg)

Lanes 1-3 : Merged signal (red and green). Green - ab218242 observed at 100,75 kDa. Red - loading control ab8245 observed at 36 kDa.

ab218242 Anti-ADAM9 antibody [EPR21924-164] was shown to specifically react with ADAM9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265484 (knockout cell lysate ab257814) was used. Wild-type and ADAM9 knockout samples were subjected to SDS-PAGE. ab218242 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ADAM9 antibody [EPR21924-164] (<a href='/products/primary-antibodies/adam9-antibody-epr21924-164-ab218242'>ab218242</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ADAM9 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ADAM9 knockout HeLa cell line (<a href='/products/cell-lines/human-adam9-knockout-hela-cell-line-ab265484'>ab265484</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 91 kDa

Observed band size: 100 kDa,75 kDa

false

Sanger Sequencing - Human ADAM9 knockout HeLa cell lysate (AB257814)

Sanger seq

Unknown

Sanger Sequencing - Human ADAM9 knockout HeLa cell lysate (AB257814)

Homozygous : 1 bp insertion in exon 1

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

疾病

Adenocarcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称

ADAM9

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

合子性

Homozygous

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ADAM9 also known as Meltrin gamma is a member of the ADAM (a disintegrin and metalloproteinase) family characterized by both proteolytic and adhesive properties. The ADAM9 protein weighs approximately 84 kDa and is expressed in various tissues including the brain throat and lungs. It plays an important role in cellular processes such as cell-cell and cell-matrix interactions due to its disintegrin domain.

Biological function summary

ADAM9 participates in numerous essential cellular functions particularly in cell surface protein shedding and proteolytic activities. It is part of the protease complex involved in shedding epidermal growth factor receptor (EGFR) ligands. This action impacts cell signaling pathways that contribute to processes such as cell proliferation migration and invasion.

Pathways

Many studies link ADAM9 to pathways involving cell signal modulation and extracellular matrix remodeling. It participates in the EGFR signaling pathway significantly affecting cell behavior. ADAM9 interacts with other proteins such as integrins which facilitate changes in cell dynamics and communication.

Evidence implicates ADAM9 in several cancers including breast cancer and prostate cancer. It plays a role in cancer development by promoting cell invasion and metastasis. ADAM9 is also associated with Alzheimer's disease through interactions with proteins like APP (amyloid precursor protein) suggesting its involvement in neuronal degradation and plaque formation.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

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人ADAM9 knockout HeLa cell裂解物

Human ADAM9 knockout HeLa cell lysate

Western blot - Human ADAM9 knockout HeLa cell lysate (AB257814)

Sanger Sequencing - Human ADAM9 knockout HeLa cell lysate (AB257814)