AB257337

人ACTN1 (alpha Actinin) knockout HeLa cell裂解物

Name: 人ACTN1 (alpha Actinin) knockout HeLa cell lysate (ab257337)| Abcam中文官网
Brand: Abcam Limited
SKU: AB257337
Price: 6206 CNY
Availability: InStock

Human ACTN1 (alpha Actinin) knockout HeLa cell lysate

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ACTN1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2.

查看别名

ACTN1_HUMAN, Actinin alpha 1, Alpha-actinin cytoskeletal isoform, Alpha-actinin-1, BDPLT15, F-actin cross-linking protein, FLJ40884, FLJ54432, Non-muscle alpha-actinin-1, actinin 1 smooth muscle

2 Images

Lab

Western blot - Human ACTN1 (alpha Actinin) knockout HeLa cell lysate (AB257337)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : ACTN1 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab68194 observed at 103 kDa. Red - loading control ab8245 observed at 37 kDa.

ab68194 Anti-alpha Actinin/ACTN1 antibody [EP2527Y] was shown to specifically react with alpha Actinin/ACTN1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265610 (knockout cell lysate ab257337) was used. Wild-type and alpha Actinin/ACTN1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab68194 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-alpha Actinin/ACTN1 antibody [EP2527Y] - Loading Control (<a href='/products/primary-antibodies/alpha-actinin-actn1-antibody-ep2527y-loading-control-ab68194'>ab68194</a>) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ACTN1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ACTN1 (alpha Actinin) knockout HeLa cell line (<a href='/products/cell-lines/human-actn1-alpha-actinin-knockout-hela-cell-line-ab265610'>ab265610</a>)

Predicted band size: 103 kDa

Observed band size: 103 kDa

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Sanger Sequencing - Human ACTN1 (alpha Actinin) knockout HeLa cell lysate (AB257337)

Sanger seq

Unknown

Sanger Sequencing - Human ACTN1 (alpha Actinin) knockout HeLa cell lysate (AB257337)

Homozygous : 4 bp deletion in exon2

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2.

疾病

Adenocarcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称

ACTN1

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Sanger Sequencing, Western blot

运输条件

Ambient - Can Ship with Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha Actinin-1 also known as ACTN1 or Actn 1 is a cytoskeletal protein that cross-links actin filaments in muscle and non-muscle cells. With a mass of approximately 100 kDa it is an important component of cell structures such as Z-discs in skeletal muscle. Alpha Actinin is expressed in a range of tissues including skeletal cardiac and smooth muscle as well as in various non-muscle cells. Its main role is to organize the actin cytoskeleton providing structural support and enabling cell motility.

Biological function summary

Alpha Actinin-1 helps maintain the structural integrity of cells by anchoring actin filaments and connecting them to integrins or membrane proteins. It often functions as part of a protein complex interacting with other cytoskeletal components and signaling molecules to stabilize cellular structures. In muscle cells it blends with titin and other sarcomeric proteins to assist muscle contraction. Beyond structural roles it partakes in cellular signaling pathways by interacting with signaling proteins and influencing cellular responses to external stimuli.

Pathways

Alpha Actinin-1 is involved in the regulation of the actin cytoskeleton pathway as well as the integrin signaling pathway. In these pathways it collaborates with proteins such as vinculin and paxillin modulating cell adhesion and movement. Its ability to interact with integrin-linked kinases suggests a role in transducing mechanical signals from the extracellular matrix to downstream signaling cascades.

Alpha Actinin-1 has been linked to a spectrum of muscular diseases and certain cancers. Mutations in Alpha Actinin-1 can contribute to hereditary diseases like congenital kidney disorders which affect actin filament organization in podocytes. In oncology altered expression levels of Alpha Actinin-1 can affect cell proliferation impacting tumor growth and metastasis. Its interaction with other proteins like focal adhesion kinase (FAK) underlines its involvement in these pathological conditions influencing cellular adhesion and signal transduction.

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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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人ACTN1 (alpha Actinin) knockout HeLa cell裂解物

Human ACTN1 (alpha Actinin) knockout HeLa cell lysate

Western blot - Human ACTN1 (alpha Actinin) knockout HeLa cell lysate (AB257337)

Sanger Sequencing - Human ACTN1 (alpha Actinin) knockout HeLa cell lysate (AB257337)