AB259778

人ABCG2 knockout A549 cell裂解物

Name: 人ABCG2 knockout A549 cell lysate (ab259778)| Abcam中文官网
Brand: Abcam Limited
SKU: AB259778
Price: 6405 CNY
Availability: InStock

Human ABCG2 knockout A549 cell lysate

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ABCG2 KO cell lysate available now. KO validated by Next Generation Sequencing. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift = 90%.

查看别名

ABC transporter, ABC15, ABCG2_HUMAN, ABCP, ATP binding cassette sub family G (WHITE) member 2, ATP binding cassette transporter G2, ATP-binding cassette sub-family G member 2, BCRP, BCRP 1, BMDP, Breast cancer resistance protein, CD338, CDw338, CDw338 antigen, EST157481, GOUT1, MGC102821, MRX, MXR, MXR1, Mitoxantrone resistance-associated protein, Multi drug resistance efflux transport ATP binding cassette sub family G (WHITE) member 2, Placenta specific MDR protein, Placenta-specific ATP-binding cassette transporter, UAQTL1

2 Images

Lab

Western blot - Human ABCG2 knockout A549 cell lysate (AB259778)

Lane 1 : Wild-type A549 cell lysate 20 μg
Lane 2 : ABCG2 knockout A549 cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : THP-1 cell lysate 20 μg

Lanes 1 - 4 : Merged signal (red and green). Green - ab207732 observed at 70 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab207732 was shown to react with BCRP/ABCG2 in wild-type A549 cells in Western blot with loss of signal observed in ABCG2 knockout cell line ab259773 (ABCG2 knockout cell lysate ab259778). Wild-type A549 and ABCG2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab207732 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-BCRP/ABCG2 antibody [EPR20080] (<a href='/products/primary-antibodies/bcrp-abcg2-antibody-epr20080-ab207732'>ab207732</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ABCG2 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human ABCG2 knockout A549 cell line (<a href='/products/cell-lines/human-abcg2-knockout-a549-cell-line-ab259773'>ab259773</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Predicted band size: 72 kDa

Observed band size: 70 kDa

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Lab

Next Generation Sequencing - Human ABCG2 knockout A549 cell lysate (AB259778)

X = 1 bp deletion

关键信息

细胞类型

A549

种属

Human

组织

Lung

敲除验证

Next Generation Sequencing

突变描述

Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift = 90%

疾病

Carcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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产品详情

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称

ABCG2

基因编辑类型

Knockout

基因编辑方法

CRISPR technology

敲除验证

Next Generation Sequencing

运输条件

Ambient - Can Ship with Ice

质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 1 US: 1

贴壁/悬浮

Adherent

性别

Male

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

人ABCG2 knockout A549 cell裂解物

Human ABCG2 knockout A549 cell lysate

Western blot - Human ABCG2 knockout A549 cell lysate (AB259778)

Next Generation Sequencing - Human ABCG2 knockout A549 cell lysate (AB259778)