HL-60全细胞裂解物

• WB

Project

Western blot - HL-60 whole cell lysate (AB7914)

ab26483 consistently detects a band at 55-60 kDa but we also see bands of ~35 and 70 kDa that appear with varying intensity in some of our extracts. We cannot find an explanation for this and are unsure of the nature of these crossreacting bands.

All lanes:

Western blot - Anti-Cdc20 antibody (<a href='/products/primary-antibodies/cdc20-antibody-ab26483'>ab26483</a>) at 1 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (<a href='/products/unavailable/raji-human-burkitt-s-lymphoma-cell-line-whole-cell-lysate-ab7908'>ab7908</a>) at 20 µg

Lane 2:

Western blot - THP-1 whole cell lysate (<a href='/products/cell-lysates/thp-1-whole-cell-lysate-ab7913'>ab7913</a>) at 20 µg

Lane 3:

Western blot - HEK-293 whole cell lysate (<a href='/products/cell-lysates/hek-293-whole-cell-lysate-ab7902'>ab7902</a>) at 20 µg

Lane 4:

Western blot - HL-60 whole cell lysate (ab7914) at 20 µg

Secondary

All lanes:

IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

Predicted band size: 55 kDa

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