Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

HeLa全细胞裂解物(ab150035)

Submit a review Q&A (1)References (1)
Western blot - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)
  • SDS-PAGE - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)
  • Western blot - HeLa whole cell lysate (ab150035)

概述

  • 产品名称

    HeLa全细胞裂解物
    参阅全部 HeLa (Human epithelial carcinoma cell line) 细胞裂解液

  • 常规说明

    We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.

     

    This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT).

  • 经测试应用

    适用于: WBmore details

性能

  • Mycoplasma free

    Yes

  • 形式

    Liquid

  • 存放说明

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.50
    Constituent: 10% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
  • Concentration information loading...

  • 裂解物说明

    This HeLa whole cell was prepared using a standard whole cell lysate protocol. 

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab150035于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.
说明
WB
Use at an assay dependent concentration.

图片

  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Observed band size: 42 kDa why is the actual band size different from the predicted?

  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Observed band size: 45 kDa why is the actual band size different from the predicted?

  • SDS-PAGE - HeLa whole cell lysate (ab150035)
    SDS-PAGE - HeLa whole cell lysate (ab150035)
    All lanes  stained with Optiblot Blue (ab119211) (20 ml)

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)

    Lysates/proteins at 10 µg per lane.
  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Observed band size: 51 kDa why is the actual band size different from the predicted?

  • Western blot - HeLa whole cell lysate (ab150035)
    Western blot - HeLa whole cell lysate (ab150035)
    All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml

    Lane 1 : HeLa whole cell lysate (ab150035)
    Lane 2 : HeLa nuclear extract lysate (ab150036)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Observed band size: 60 kDa why is the actual band size different from the predicted?

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab150035？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab150035 被引用在 1 文献中.

  • Masloh S  et al. Enhancing Oral Delivery of Biologics: A Non-Competitive and Cross-Reactive Anti-Leptin Receptor Nanofitin Demonstrates a Gut-Crossing Capacity in an Ex Vivo Porcine Intestinal Model. Pharmaceutics 16:N/A (2024). PubMed: 38258126

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

Question

Dear recipient,
Does the Dkk1 antibody ab 61275 include the positive control mentioned
in the data sheet (HeLa cell lysate)? Or should I purchase it
separately?
Thank you very much for your reply.
Best regards,

Read More
Answer

Thank you for contacting us.

I can confirm that the positive control is not included into an order of the DKK antibody ab61275.

However, we do offer two products of HeLa cell lysate: ab29545 (tested for WB application) and ab150035 that can be used as positive controls for WB. For further information about both products use the provided links below.

ab29545
https://www.abcam.com/HeLa-whole-cell-lysate-ab29545.html (or use the following: https://www.abcam.com/HeLa-whole-cell-lysate-ab29545.html).


ab150035
https://www.abcam.com/HeLa-whole-cell-lysate-ab150035.html (or use the following: https://www.abcam.com/HeLa-whole-cell-lysate-ab150035.html).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com