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Tags & Cell Markers Epitope Tags HA Tag

E. coli Positive Control (Escherichia coli )全细胞裂解物(ab5395)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (3)

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Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

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概述

  • 产品名称

    E. coli Positive Control (Escherichia coli )全细胞裂解物
  • 常规说明

    This E.coli lysate contains a 41 kd recombinant protein expressing 12 commonly used Tags which are fused to the N-terminus of the protein in the order of:

    GST

    T7

    HSV

    C-Myc

    VSV-g

    Glu-Glu

    V5

    E-tag

    FLAG (DDDDK)

    S-tag

    HA

    6x His

    Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.

  • 经测试应用

    适用于: WBmore details

性能

  • Mycoplasma free

    Yes
  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    1X Loading Buffer (1X SDS-PAGE Sample Buffer: 62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue)
  • Concentration information loading...
  • 裂解物说明

    Excellent as a loading control for Western blotting. Lysate expresses 12 different epitope tags.

  • 研究领域

    • Tags & Cell Markers
    • Epitope Tags
    • HA Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Myc Tag
    • Tags & Cell Markers
    • Epitope Tags
    • DDDDK Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Glu-Glu Tag
    • Tags & Cell Markers
    • Epitope Tags
    • V5 Tag
    • Tags & Cell Markers
    • Epitope Tags
    • T7 Tag
    • Microbiology
    • Organism
    • Bacteria
    • Gram negative Bacteria
    • Escherichia coli
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • E tag
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • VSV-G Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • S Tag
    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • HSV Tag
    • Kits/ Lysates/ Other
    • Lysates
    • Whole Cell Lysates
    • Arabidopsis / Miscellaneous
  • 背景

    Escherichia coli is a gram negative bacillus that belongs to a larger group of Enterobacteriae - bacteria that inhabit the gastrointestinal tract. Although usually a harmless resident of the gut, some strains have the potential to cause serious problems, especially where there is an immature immune system or immunosuppression, or where the subtype of organism has acquired the ability to produce pathogenic toxins.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5395于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

说明
WB
Use at an assay dependent concentration.

Use 0.5 - 2.0 µl for 10 - 12% gels.

图片

  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    All lanes : Anti-6X His tag® antibody (ab9108) at 1/1000 dilution

    Lane 1 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 2 µl
    Lane 2 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 1 µl
    Lane 3 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.5 µl
    Lane 4 : E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395) at 0.25 µl
  • Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)
    Western blot - E. coli Positive Control (Escherichia coli ) Whole Cell Lysate (ab5395)

    This WB was performed using an E. coli lysate that expresses a 41kDa recombinant protein comprised of 12 commonly used epitope tags (T7, HSV, C-Myc, VSV-g, Glu-Glu, V5, E-tag, FLAG, S-tag, HA and 6xHis). This lysate is available as ab5395.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (3)

发表研究结果有使用 ab5395?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5395 被引用在 3 文献中.

  • Shokri L  et al. A Comprehensive Drosophila melanogaster Transcription Factor Interactome. Cell Rep 27:955-970.e7 (2019). PubMed: 30995488
  • Májek P  et al. Improved Coomassie Blue Dye-Based Fast Staining Protocol for Proteins Separated by SDS-PAGE. PLoS One 8:e81696 (2013). PubMed: 24278455
  • Miriam_Sans, Itzel_Vargas-Prez, Luis_Quintales, Fr Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res : 1-11 (2011). WB ; Schizosaccharomyces pombe . PubMed: 21515633

客户评价及客户问答

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1-3 of 3 Abreviews or Q&A

Question

Hey,

Is the concentration of the tagged fusion protein known? If not, do you sell any myc-tagged fusion proteins where the concentration is known?

Read More

Abcam community

Verified customer

Asked on Mar 07 2013

Answer

Thank you for contacting us.
We do not have an exact concentration for this material and do not have another lysate where the exact concentration of the myc-tagged protein is known.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

回复于 Mar 07 2013

Question

J’aurais besoin de savoir quelle est la souche d’E.Coli utilisée pour le lysat ab5395 (concernant le lysat ab2431 la souche utilisé est BL21).

Merci beaucoup pour votre retour

Cordialement

Read More

Abcam community

Verified customer

Asked on May 17 2012

Answer

J'espère que vous allez bien!

Je vous écrit parce que j'ai finalement reçu les informations par rapportà la souche du lysat ab5395. Le laboratoire m'a confirmé que c'est la souche BL21. Je suis très désolée pour le retard de cette réponse. J'espère que cette information vous est quand-même utile.

Aussi, est-ce que vous avez eu des bons résultats avec le nouveau tube de ab2431?

Encore une fois toutes mes excuses pour ce délais de réponse. N'hésitez pas à nous recontacter si vous avez des autres questions.

Read More

Abcam Scientific Support

回复于 May 17 2012

Question

Do you have the sequence of each of the tags contained in the recombinant protein?

Read More

Abcam community

Verified customer

Asked on Mar 06 2006

Answer

Thank you for your enquiry. Here is the sequence information I have for this product: N' GST - T7 - HSV - C-MYC - VSV-g - GluGlu - a tubulin - V5 - E-tag - Flag - S-tag - HA - 6xHIS C' T7 = CMASMTGGQQMG HSV = QPELAPEDPEDC CMYC = EQKLISEEDLC HA = YPYDVPDYAC VSV-g = YTDIEMNRLGK GluGlu = EYMPME E-tag = GAPVPYPDPLEPR FLAG = DYKDDDDK V5 = TSTLPVDTTDWIEGETYK 6xHIS = HHHHHH I hope this information helps. Please do not hesitate to contact us again if you need anything further.

Read More

Abcam Scientific Support

回复于 Mar 06 2006

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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