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AB265122

人TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line

Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line

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TFAP2A KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.

查看别名

AP-2, AP-2 transcription factor, AP2-alpha, AP2A_HUMAN, AP2TF, Activating enhancer-binding protein 2-alpha, Activator protein 2, BOFS, FLJ51761, TFAP 2, TFAP 2A, Transcription factor AP 2 alpha (activating enhancer binding protein 2 alpha), Transcription factor AP-2-alpha

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Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (AB265122)
  • WB

Lab

Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (AB265122)

Lanes 1- 2 : Merged signal (red and green). Green - ab108311 observed at 48 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab108311 was shown to react with Transcription factor AP-2-alpha in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265122 (knockout cell lysate ab257736) was used. Wild-type HeLa and TFAP2A knockout HeLa cell lysates were subjected to SDS-PAGE. ab108311 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (<a href='/products/primary-antibodies/transcription-factor-ap-2-alpha-antibody-epr26882-ab108311'>ab108311</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TFAP2A knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (ab265122)

Predicted band size: 122 kDa,125 kDa,32 kDa,39 kDa,40 kDa,41 kDa,42 kDa,45 kDa,48 kDa,74 kDa,77 kDa,83 kDa,97 kDa

Observed band size: 100 kDa,120 kDa,125 kDa,150 kDa,31 kDa,40 kDa,41 kDa,42 kDa,45 kDa,48 kDa,50 kDa,70 kDa,74 kDa,75 kDa,77 kDa,95 kDa

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Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (AB265122)
  • Sanger seq

Unknown

Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (AB265122)

Allele-1 : 1 bp insertion in exon 2.

Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (AB265122)
  • Sanger seq

Lab

Sanger Sequencing - Human TFAP2A (Transcription factor AP-2-alpha) knockout HeLa cell line (AB265122)

Allele-2 : Insertion of the selection cassette in exon 2.

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

形式

Liquid

form

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2

疾病

Adenocarcinoma

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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规格

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性能和储存信息

基因名称
TFAP2A
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing, Western blot
运输条件
Dry Ice
推荐的短期储存条件
-196°C
推荐的长期储存条件
-196°C
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处理步骤

初始处理指南

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

传代培养指南
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
培养基

DMEM (High Glucose) + 10% FBS

低温储藏试剂

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Transcription factor AP-2-alpha also known as TFAP2A or AP-2 is a protein acting as an important regulator in cellular processes. It weighs approximately 52 kDa. TFAP2A is known for its role in binding specific DNA sequences to regulate target gene transcription. It expresses highly in neural crest-derived tissues as well as in the placenta breast and skin. This transcription factor plays an essential role in embryonic development cell proliferation and differentiation.
Biological function summary

TFAP2A contributes significantly to the regulation of gene expression involved in essential developmental and cellular processes. It acts as part of a complex with other transcription factors allowing it to coordinate the expression of a variety of genes linked to cell growth and apoptosis. AP-2 also regulates genes involved in neural development and skin morphogenesis. Its interaction with other factors such as AP-2γ and AP-2β enhances its functionality indicating a complex mechanism of action.

Pathways

TFAP2A engages in critical signalling circuits such as the MAPK and Wnt pathways which are essential for cell cycle regulation and embryonic development. Within these pathways related proteins like AP-2γ and AP-594 interact with TFAP2A further illustrating its role in signal transduction. These relationships highlight the importance of TFAP2A in orchestrating cellular responses to growth signals and environmental cues.

TFAP2A has links to a range of pathological conditions including cancer and neural crest-related disorders. In melanoma for example TFAP2A's regulatory role affects genes associated with tumor growth and metastasis. The protein's interaction with other transcription factors like AP-647 may influence cancer progression and therapy response. Additionally the dysregulation of TFAP2A affects neural crest development potentially leading to congenital disorders. Understanding these interactions assists in unraveling the molecular basis of such diseases and informs therapeutic strategies.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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靶点信息

See full target information TFAP2A
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搭配使用产品

Primary Antibodies

AB108311

Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)]

BOND RX™ Validated|Advanced Validation|RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Transcription factor AP-2-alpha antibody [EPR2688(2)] (AB108311)

  • 5
  • 2
Cell Lines & Lysates

AB265841

Human TFG (TRK fused gene) knockout HeLa cell line

Advanced Validation

Western blot - Human TFG (TRK fused gene) knockout HeLa cell line (AB265841)

  • 0
  • 0
Primary Antibodies

AB109503

Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control

RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transferrin antibody [EPR2932(2)] - Serum Loading Control (AB109503)

  • 5
  • 2
Proteins & Peptides

AB195163

Recombinant Human ZMYND8 protein

SDS-PAGE - Recombinant Human ZMYND8 protein (AB195163)

  • 0
  • 0

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