JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB265637

人PPP2R5E knockout HeLa cell line

Human PPP2R5E knockout HeLa cell line

Be the first to review this product! Submit a review

|

(0 Publication)

PPP2R5E KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.

查看别名

2A5E_HUMAN, Epsilon isoform of regulatory subunit B56 protein phosphatase 2A, PP2A B subunit B' epsilon, PP2A B subunit B' epsilon isoform, PP2A B subunit B56 epsilon, PP2A B subunit B56 epsilon isoform, PP2A B subunit PR61 epsilon, PP2A B subunit PR61 epsilon isoform, PP2A B subunit R5 epsilon, PP2A B subunit R5 epsilon isoform, PP2A B subunit isoform B''-epsilon, PP2A B subunit isoform B'-epsilon, PP2A B subunit isoform B56-epsilon, PP2A B subunit isoform PR61-epsilon, PP2A B subunit isoform R5-epsilon, PPP2R5E, Protein phosphatase 2 regulatory subunit B (B56) epsilon isoform, Protein phosphatase 2 regulatory subunit B' epsilon, Protein phosphatase 2 regulatory subunit B' epsilon isoform, Regulatory subunit B of protein phosphatase 2 epsilon isoform, Serine/threonine protein phosphatase 2A 56 kDa regulatory subunit epsilon, Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit epsilon isoform

4 Images
Western blot - Human PPP2R5E knockout HeLa cell line (AB265637)
  • WB

Lab

Western blot - Human PPP2R5E knockout HeLa cell line (AB265637)

Lanes 1-4 : Merged signal (red and green). Green - ab198290 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab198290 Anti-PPP2R5E antibody [EPR17146] was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198290 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17146] (<a href='/products/primary-antibodies/ppp2r5e-antibody-epr17146-ab198290'>ab198290</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PPP2R5E knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PPP2R5E knockout HeLa cell line (ab265637)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Western blot - Human PPP2R5E knockout HeLa cell line (AB265637)
  • WB

Lab

Western blot - Human PPP2R5E knockout HeLa cell line (AB265637)

Lanes 1-4 : Merged signal (red and green). Green - ab198500 observed at 55 kDa. Red - loading control ab8245 observed at 37 kDa.

ab198500 Recombinant Anti-PPP2R5E antibody [EPR17147] - C-terminal was shown to specifically react with PPP2R5E in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265637 (knockout cell lysate ab258135) was used. Wild-type and PPP2R5E knockout samples were subjected to SDS-PAGE. ab198500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 5000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PPP2R5E antibody [EPR17147] - C-terminal (<a href='/products/primary-antibodies/ppp2r5e-antibody-epr17147-c-terminal-ab198500'>ab198500</a>) at 1/5000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PPP2R5E knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PPP2R5E knockout HeLa cell line (ab265637)

Lane 3:

HEK-293T cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 55 kDa

Observed band size: 55 kDa

false

Sanger Sequencing - Human PPP2R5E knockout HeLa cell line (AB265637)
  • Sanger seq

Unknown

Sanger Sequencing - Human PPP2R5E knockout HeLa cell line (AB265637)

Allele-2 : Insertion of the selection cassette in exon 2.

Sanger Sequencing - Human PPP2R5E knockout HeLa cell line (AB265637)
  • Sanger seq

Unknown

Sanger Sequencing - Human PPP2R5E knockout HeLa cell line (AB265637)

Allele-1 : 1 bp deletion in exon 2.

关键信息

细胞类型

HeLa

种属

Human

组织

Cervix

形式

Liquid

form

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2

疾病

Adenocarcinoma

style
left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

产品详情

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

规格

{ "values": { "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab265637-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab255928 Human wild-type HeLa cell line", "number":"AB265637-CMP02" }, { "size":"1 x 1000000 Cells/vial", "name":"ab265637 Human PPP2R5E knockout HeLa cell line", "number":"AB265637-CMP01" } ] }, "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab265637-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab265637 Human PPP2R5E knockout HeLa cell line", "number":"AB265637-CMP01" }, { "size":"1 x 1000000 Cells/vial", "name":"ab255928 Human wild-type HeLa cell line", "number":"AB265637-CMP02" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab265637-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab265637 Human PPP2R5E knockout HeLa cell line", "number":"AB265637-CMP01" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab265637-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab265637 Human PPP2R5E knockout HeLa cell line", "number":"AB265637-CMP01", "productcode":"" } ] } } }
style
left-column

性能和储存信息

基因名称
PPP2R5E
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing, Western blot
运输条件
Dry Ice
推荐的短期储存条件
-196°C
推荐的长期储存条件
-196°C
style
left-column

处理步骤

初始处理指南

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

传代培养指南
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
培养基

DMEM (High Glucose) + 10% FBS

低温储藏试剂

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

style
left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The protein PPP2R5E also known as B56 epsilon is a regulatory subunit of protein phosphatase 2A (PP2A). It has an approximate mass of 72 kDa. PPP2R5E expresses across various tissues including the brain heart and skeletal muscle. The subunit associates with the core PP2A enzyme influencing substrate specificity and activity of PP2A holoenzyme.
Biological function summary

PPP2R5E plays a role in regulating cell growth and division. It is a part of the PP2A holoenzyme complex acting as a critical modulatory component. By associating with the PP2A core enzyme PPP2R5E contributes to the dephosphorylation process of many substrates. These substrates are vital for maintaining cellular functions such as signal transduction and cell cycle progression.

Pathways

PPP2R5E takes part in cell signaling and apoptotic pathways. It has substantial involvement in the MAPK signaling pathway where it modulates the activity of related kinases. PPP2R5E interacts with proteins like RAF1 influencing the pathway's function. Additionally it also participates in the Wnt signaling pathway where its regulatory role impacts cellular differentiation and proliferation.

PPP2R5E shows links to cancer and neurodegenerative diseases. Alterations in its expression or function may lead to oncogenesis due to its role in controlling cell proliferation signals. The dysregulation of PP2A activity potentially through PPP2R5E has associations with Alzheimer's disease. In cancer PPP2R5E interacts with proteins such as c-Myc which contributes to tumorigenesis and progression.
style
left-column
style
left-column

质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

style
left-column

产品实验方案

style
left-column
style
left-column
style
right-column

搭配使用产品

Proteins & Peptides

AB313361

Recombinant Human WFDC2 Protein (His tag)

Mass Spectrometry - Recombinant Human WFDC2 Protein (His tag) (AB313361)

  • 0
  • 0
Proteins & Peptides

AB95933

Recombinant human XIAP protein

Functional Studies - Recombinant human XIAP protein (AB95933)

  • 0
  • 0
Primary Antibodies

AB198500

Anti-PPP2R5E antibody [EPR17147] - C-terminal

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-PPP2R5E antibody [EPR17147] - C-terminal (AB198500)

  • 0
  • 0
Proteins & Peptides

AB217407

Recombinant human Vitronectin/S-Protein (Animal Free)

  • 0
  • 0

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com