人NUCB1 knockout HEK-293T cell line
Human NUCB1 knockout HEK-293T cell line
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NUCB1 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp deletion in exon 3.
查看别名
CALNUC, FLJ40471, NUCB1_HUMAN, Nucleobindin-1
- Sanger seq
Unknown
Sanger Sequencing - Human NUCB1 knockout HEK-293T cell line (AB266299)
Allele-1 : 2 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human NUCB1 knockout HEK-293T cell line (AB266299)
Allele-2 : 1 bp insertion in exon 3.
产品详情
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
运输条件
推荐的短期储存条件
推荐的长期储存条件
处理步骤
初始处理指南
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
传代培养指南
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
培养基
DMEM (High Glucose) + 10% FBS
低温储藏试剂
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
NUCB1 participates in the regulation of cellular calcium homeostasis and signaling pathways. It is not a part of a multi-protein complex but it acts as a molecular chaperone influencing the stability and function of other proteins within the cell. NUCB1 also plays a role in secretory pathways within the cell which is relevant to its expression in endocrine tissues. Its regulatory functions in calcium signaling are critical for cellular activities such as secretion and cell division.
Pathways
NUCB1 interacts with cellular signaling mechanisms particularly those related to intracellular calcium dynamics. It is involved in pathways like the calcium signaling pathway and the insulin signaling pathway. Within these pathways NUCB1 associates with proteins such as calmodulin which also binds calcium and contributes to signal transduction. The protein modulates activities within these pathways affecting how cells respond to external signals.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
Complementary Products
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com