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AB266519

人MAPK3 (ERK1) knockout HEK-293T cell line

Human MAPK3 (ERK1) knockout HEK-293T cell line

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(1 Publication)

MAPK3 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.

查看别名

ERK-1, ERT 2, Extracellular signal related kinase 1, Extracellular signal-regulated kinase 1, HGNC6877, HS44KDAP, HUMKER1A, Insulin-stimulated MAP2 kinase, MAP Kinase, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK, MAPK 1, MGC20180, MK03_HUMAN, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 1, Mitogen-activated protein kinase 3, OTTHUMP00000174538, OTTHUMP00000174541, PRKM 3, Protein Kinase Mitogen Activated 3, p44-ERK1, p44-MAPK

4 Images
Western blot - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)
  • WB

Lab

Western blot - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)

Lanes 1- 2 : Merged signal (red and green). Green - ab109282 observed at 43 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab109282 was shown to react with ERK1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266519 (knockout cell lysate ab257099) was used. Wild-type HEK-293T and MAPK3 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109282 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4° at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERK1 antibody [EP4967] (<a href='/products/primary-antibodies/erk1-antibody-ep4967-ab109282'>ab109282</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

MAPK3 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK3 (ERK1) knockout HEK-293T cell line (ab266519)

Predicted band size: 43 kDa

Observed band size: 43 kDa

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Western blot - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)
  • WB

Lab

Western blot - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)

Lanes 1- 2 : Merged signal (red and green). Green - ab32537 observed at 43 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab32537 was shown to react with ERK1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266519 (knockout cell lysate ab257099) was used. Wild-type HEK-293T and MAPK3 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32537 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4° at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ERK1 antibody [Y72] (<a href='/products/primary-antibodies/erk1-antibody-y72-ab32537'>ab32537</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

MAPK3 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human MAPK3 (ERK1) knockout HEK-293T cell line (ab266519)

Predicted band size: 43 kDa

Observed band size: 43 kDa

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Sanger Sequencing - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)
  • Sanger seq

Unknown

Sanger Sequencing - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)

Homozygous : 1 bp deletion in exon1

Cell Culture - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)
  • Cell Culture

Lab

Cell Culture - Human MAPK3 (ERK1) knockout HEK-293T cell line (AB266519)

Representative images MAPK3 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS M5000 microscope.

关键信息

细胞类型

HEK-293T

种属

Human

组织

Kidney

形式

Liquid

form

敲除验证

Sanger Sequencing,Western blot

突变描述

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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规格

{ "values": { "2x1000000Cellsvial": { "sellingSize": "2 x 1000000 Cells/vial", "publicAssetCode":"ab266519-2x1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab266519 Human MAPK3 (ERK1) knockout HEK-293T cell line", "number":"AB266519-CMP01" }, { "size":"1 x 1000000 Cells/vial", "name":"ab255449 Human wild-type HEK-293T cell line", "number":"AB266519-CMP02" } ] }, "1000000Cellsvial": { "sellingSize": "1000000 Cells/vial", "publicAssetCode":"ab266519-1000000Cells_vial", "assetComponentDetails": [ { "size":"1 x 1000000 Cells/vial", "name":"ab266519 Human MAPK3 (ERK1) knockout HEK-293T cell line", "number":"AB266519-CMP01", "productcode":"" } ] } } }
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性能和储存信息

基因名称
MAPK3
基因编辑类型
Knockout
基因编辑方法
CRISPR technology
敲除验证
Sanger Sequencing, Western blot
合子性
Homozygous
运输条件
Dry Ice
推荐的短期储存条件
-196°C
推荐的长期储存条件
-196°C
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处理步骤

初始处理指南

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

传代培养指南
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
培养基

DMEM (High Glucose) + 10% FBS

低温储藏试剂

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

ERK1 also known as MAPK3 is an extracellular signal-regulated kinase involved in transmitting signals from the cell surface to the nucleus. This protein has a molecular mass of about 44 kDa. ERK1 expresses in various tissue types with higher expression in the brain heart and skeletal muscle. Researchers often study ERK1 in the context of its role in cellular signaling due to its involvement in critical regulatory functions.
Biological function summary

ERK1 plays a significant role in cell cycle regulation differentiation and proliferation. It forms part of the MAPK signaling cascade becoming activated through a phosphorylation event. In its activated form ERK1 translocates to the nucleus where it phosphorylates target substrates. ERK1 often functions in conjunction with its homolog ERK2 to mediate these cellular processes marking it as an essential player in growth factor signaling.

Pathways

ERK1 functions primarily within the MAPK/ERK signaling pathway a major conduit for transmitting proliferative signals from growth factor receptors. ERK1 interacts with proteins like MEK1/2 which phosphorylate and activate ERK1 in response to extracellular stimuli. Another critical pathway involving ERK1 is the Ras-Raf-MEK-ERK cascade which regulates various cellular outcomes. This connection to the Ras family highlights its importance in signal transduction and reinforces its position in critical cellular processes.

Aberrant activation of ERK1 connects to diseases such as cancer and cardiovascular disorders. In cancer the dysregulation of the MAPK/ERK pathway often through mutations affecting Ras or Raf proteins leads to uncontrolled cell proliferation. ERK1's involvement in cardiovascular diseases links to its role in hypertrophic signaling in cardiac cells where altered ERK1 activity can contribute to pathological cardiac remodeling. Understanding these interactions can aid in developing therapeutic strategies targeting the MAPK/ERK signaling pathway.
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质量控制

STR 分析

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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细胞培养

生物安全等级

EU: 2 US: 2

贴壁/悬浮

Adherent

性别

Female

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产品实验方案

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靶点信息

See full target information MAPK3
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 19:396 PubMed38982418

2024

SP1 regulates BMSC osteogenic differentiation through the miR-133a-3p/MAPK3 axis : SP1 regulates osteogenic differentiation of BMSCs.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Zhong,Yehai Sun,Cong Wang,Runzhi Liu,Wenjuan Ru,Wei Dai,Ting Xiong,Aimin Zhong,Shundong Li
View all publications
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搭配使用产品

Primary Antibodies

AB76299

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [EP197Y] (AB76299)

  • 0
  • 0
Assay Kits

AB176640

ERK1/2 (pT202/Y204) ELISA Kit

SimpleStep

ELISA - ERK1/2 (pT202/Y204) ELISA Kit (AB176640)

  • 4
  • 1
Proteins & Peptides

AB116536

Recombinant human ERK1 protein

SDS-PAGE - Recombinant human ERK1 protein (AB116536)

  • 0
  • 0
Proteins & Peptides

AB95933

Recombinant human XIAP protein

Functional Studies - Recombinant human XIAP protein (AB95933)

  • 0
  • 0

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