人LAPTM4A knockout HEK-293T cell line
Human LAPTM4A knockout HEK-293T cell line
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LAPTM4A KO cell line available to order. KO validated. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
查看别名
Golgi 4-transmembrane-spanning transporter MTP, HUMORF13, KIAA0108, LAP4A_HUMAN, LAPTM4, LAPTM4A, Lysosomal associated protein transmembrane 4 alpha, Lysosomal protein transmembrane 4 alpha, Lysosomal-associated transmembrane protein 4A, MBNT, MTRP, Membrane nucleoside transporter
- Sanger seq
Unknown
Sanger Sequencing - Human LAPTM4A knockout HEK-293T cell line (AB266686)
Homozygous : 7 bp deletion in exon 1
产品详情
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
规格
性能和储存信息
基因名称
基因编辑类型
基因编辑方法
敲除验证
合子性
运输条件
推荐的短期储存条件
推荐的长期储存条件
处理步骤
初始处理指南
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
传代培养指南
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
培养基
DMEM (High Glucose) + 10% FBS
低温储藏试剂
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
补充信息
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein contributes to cell comprehension by facilitating lysosomal function and maintaining cellular homeostasis. LAPTM4A participates in the regulation of proliferation and survival often forming a complex with other lysosomal proteins for transmitting signals related to these processes. It impacts cellular signaling pathways by interacting with certain membrane receptors and transporting them to lysosomes for degradation or recycling.
Pathways
LAPTM4A is an integral part of endocytic and vesicular transport pathways. It influences receptor-mediated endocytosis that directs cell surface proteins to lysosomes for degradation or recycling. In these pathways LAPTM4A interacts with other proteins like c-MET a receptor tyrosine kinase that plays a part in signal transduction processes linked to cell growth.
质量控制
STR 分析
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
细胞培养
生物安全等级
EU: 2 US: 2
贴壁/悬浮
Adherent
性别
Female
搭配使用产品
Abcam Product Promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com