人ERCC4 knockout HeLa cell line

• WB

Lab

Western blot - Human ERCC4 knockout HeLa cell line (AB280068)

False colour image of Western blot : Anti-XPF antibody staining at 1/2000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab76948 was shown to bind specifically to XPF. A band was observed at 105 kDa in wild-type HeLa cell lysates with no signal observed at this size in ercc4 knockout cell line ab280068 (knockout cell lysate ab280127). To generate this image wild-type and ercc4 knockout HeLa cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-XPF antibody (<a href='/products/primary-antibodies/xpf-antibody-ab76948'>ab76948</a>) at 1/2000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ercc4 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ERCC4 knockout HeLa cell line (ab280068)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Predicted band size: 104 kDa

Observed band size: 105 kDa

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• WB

Lab

Western blot - Human ERCC4 knockout HeLa cell line (AB280068)

All lanes:

Western blot - Anti-XPF antibody (<a href='/products/primary-antibodies/xpf-antibody-ab227712'>ab227712</a>) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ERCC4 knockout HeLa cell lysate (<a href='/products/cell-lysates/human-ercc4-knockout-hela-cell-lysate-ab280127'>ab280127</a>)

Lane 2:

Western blot - Human ERCC4 knockout HeLa cell line (ab280068)

Lane 2:

ercc4 knockout HeLa cell lysate at 20 µg

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 110 kDa

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• WB

Supplier Data

Western blot - Human ERCC4 knockout HeLa cell line (AB280068)

False colour image of Western blot : Anti-ercc4 antibody staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to ercc4. A band was observed at 105 kDa in wild-type HeLa cell lysates with no signal observed at this size in ercc4 knockout cell line ab280068 (knockout cell lysate ab280127). To generate this image, wild-type and ercc4 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Human ERCC4 knockout HeLa cell line (ab280068)

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• Sanger seq

Supplier Data

Sanger Sequencing - Human ERCC4 knockout HeLa cell line (AB280068)