人CD1C knockout Jurkat cell line (ab273867)
概述
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产品名称
人CD1C knockout Jurkat cell line -
Parental Cell Line
Jurkat -
Organism
Human -
Mutation description
Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift: 100% -
Passage number
<20 -
Knockout validation
Next Generation Sequencing (NGS) -
经测试应用
适用于: Next Generation Sequencing, Flow Cytmore details -
Biosafety level
1 -
常规说明
Recommended control: Human wild-type Jurkat cell line (ab271143). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
Cryopreservation cell medium: Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Culture medium: RPMI + 10% FBS
Initial handling guidelines: Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 1x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.Subculture guidelines:
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 1x105 cells/mL is recommended.
- Do not allow the cell density to exceed 3x106.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
We will provide viable cells that proliferate on revival.
性能
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Number of cells
1 x 106 cells/vial, 1 mL -
Adherent /Suspension
Suspension -
Tissue
Blood -
Cell type
T cell lymphoblast-like -
Disease
Non-Hodgkin Lymphoma -
Gender
Male -
Mycoplasma free
Yes -
存放说明
Shipped on Dry Ice. Store in liquid nitrogen. -
存储溶液
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
研究领域
靶标
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功能
Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. -
组织特异性
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. -
序列相似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
细胞定位
Cell membrane. Endosome membrane. Subject to intracellular trafficking between the cell membrane and endosomes. - Information by UniProt
相关产品
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KO cell lysates
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab273867于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Next Generation Sequencing |
Use at an assay dependent concentration.
|
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| Flow Cyt |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Next Generation Sequencing
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. |
图片
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Next Generation Sequencing - Human CD1C knockout Jurkat cell line (ab273867)1 bp deletion after Phe111 of the WT protein
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Flow Cytometry - Human CD1C knockout Jurkat cell line (ab273867)Flow cytometry overlay histogram histogram showing wild-type (green line) and CD1C knockout (red line) cells (ab273867) stained with ab190305. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab190305) (1x106 in 100 µl at 0.2 µg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was mouse IgG1, kappa (ab170190) used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line; CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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Flow Cytometry - Human CD1C knockout Jurkat cell line (ab273867)Flow cytometry overlay histogram histogram showing wild-type (green line) and CD1C knockout Jurkat cells (ab273867) stained with ab273049 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab273049) (1x106 in 100 µl at 0.2 µg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type Jurkat - black line; CD1C knockout Jurkat - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.
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Next Generation Sequencing - Human CD1C knockout Jurkat cell line (ab273867)Knockout achieved by CRISPR/Cas9; X = 1 bp deletion; Frameshift: 100%
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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