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AB152163

Cell Lysis Buffer

Cell Lysis Buffer

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(35 Publications)

Cell Lysis Buffer (ab152163) is 10X stock buffer for cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells under non-denatured conditions. Suitable for western blot and IP applications.

- Proven performance: cited in over 30 publications

关键信息

应用

WB, IP

applications

形式

Liquid

form

存储溶液

pH: 7.5 Constituents: 10% Triton X-100, 8.8% Sodium chloride, 3.15% Tris HCl, 1.12% Sodium pyrophosphate decahydrate, 0.38% EGTA, 0.29% Tetrasodium ethylene diamine tetraacetate, 0.216% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate, 0.19% Sodium orthovanadate, 0.001% Leupeptin

storage-buffer

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

产品详情

ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

Recommended for preparing samples for use in western blotting and immunoprecipitation. For use with adherent or suspension cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
储存信息
Stable for 12 months at -20°C

产品实验方案

靶点信息

文献 (35)

Recent publications for all applications. Explore the full list and refine your search

Journal of cosmetic dermatology 24:e70228 PubMed40353553

2025

Platelet-Released Growth Factors (PRGFs) Activate NRF2-ARE and Modulate Inflammatory Response in an NRF2-Dependent Manner in Primary Human Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Stein,Nicole Böttcher,Mersedeh Tohidnezhad,Athanassios Fragoulis,Andreas Bayer,Hannes Klump,Jens M Baron,Thomas Pufe

International journal of molecular sciences 26: PubMed39859163

2025

Alterations in Autophagic Function and Endoplasmic Reticulum Stress Markers in the Peripheral Blood Mononuclear Cells of Patients on Hemodialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Chih Liu,Ming-Yin Wu,Paik Seong Lim

Science advances 10:eadp7022 PubMed39485841

2024

Reprogramming cellular senescence in the tumor microenvironment augments cancer immunotherapy through multifunctional nanocrystals.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Yinglu Chen,Hui Fang,Kai Xiao,Ziping Wu,Xiaochun Xie,Jie Liu,Fangman Chen,Yi He,Liang Wang,Chao Yang,Renjun Pei,Dan Shao

Macromolecular bioscience 24:e2300414 PubMed38035771

2023

In Vitro and In Vivo Evaluation of 3D Printed Poly(Ethylene Glycol) Dimethacrylate-Based Photocurable Hydrogel Platform for Bone Tissue Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Janitha M Unagolla,Bipin Gaihre,Ambalangodage C Jayasuriya

Life sciences 321:121598 PubMed36963720

2023

Collagen matricryptin promotes cardiac function by mediating scar formation.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel A Grilo,Sirin N Cakir,Patti R Shaver,Rugmani P Iyer,Kaitlin Whitehead,Joseph M McClung,Ali Vahdati,Lisandra E de Castro Brás

International journal of molecular sciences 23: PubMed36012289

2022

Increased Epidermal Nerve Growth Factor without Small-Fiber Neuropathy in Dermatomyositis.

Applications

Unspecified application

Species

Unspecified reactive species

Lai-San Wong,Chih-Hung Lee,Yu-Ta Yen

Cells 10: PubMed33919736

2021

Periostin Short Fragment with Exon 17 via Aberrant Alternative Splicing Is Required for Breast Cancer Growth and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Ikeda-Iwabu,Yoshiaki Taniyama,Naruto Katsuragi,Fumihiro Sanada,Nobutaka Koibuchi,Kana Shibata,Kenzo Shimazu,Hiromi Rakugi,Ryuichi Morishita

Journal of virology 95: PubMed33536170

2021

Nonsegmented Negative-Sense RNA Viruses Utilize -Methyladenosine (mA) as a Common Strategy To Evade Host Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Mijia Lu,Miaoge Xue,Hai-Tao Wang,Elizabeth L Kairis,Sadeem Ahmad,Jiangbo Wei,Zijie Zhang,Qinzhe Liu,Yuexiu Zhang,Youling Gao,Dominique Garcin,Mark E Peeples,Amit Sharma,Sun Hur,Chuan He,Jianrong Li

Scientific reports 11:5687 PubMed33707502

2021

Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Mostafa EzEldeen,Burak Toprakhisar,Denise Murgia,Nick Smisdom,Olivier Deschaume,Carmen Bartic,Hans Van Oosterwyck,Rafaela Vaz Sousa Pereira,Ghislain Opdenakker,Ivo Lambrichts,Annelies Bronckaers,Reinhilde Jacobs,Jennifer Patterson

BMC cardiovascular disorders 21:51 PubMed33526034

2021

CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wang,Ming Bai
View all publications
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