Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human astrocytoma labelling Pan Trk with ab246551 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on human astrocytoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PTPN12 with ab323839 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human colon.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 µg/ml). Cytoplasmic staining on human cerebrum. The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma labelling Niemann Pick C2 with ab218192 at 1/8000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on Human breast carcinoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human skin (PMID : 8092987) is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on human testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Mannose Receptor with ab313731 at 1/50 (10.0 ug/ml) . Membranous staining on immune cells in human colon. The section was incubated with ab313731 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SMARCA2 / BRM with ab307771 at 1/100 dilution (5.0 µg/ml). Nuclear staining on human cerebrum. The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SMARCA2 / BRM with ab307770 at 1/100 dilution (5.0 µg/ml). Nuclear staining on human cerebrum. The section was incubated with ab307768 at 4°C overnight (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Met (c-Met) - C-terminal with ab314588 at 1/100 (5.0 ug/ml). Positive staining on human colon cancer. The section was incubated with ab314588 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling LC3B with ab307768 at 1/100 dilution (5.0 µg/ml). Cytoplasmic staining on human glioma. The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Met (c-Met) - C-terminal with ab314588 at 1/100 (5.0 ug/ml). Positive staining on human colon. The section was incubated with ab314588 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mannose Receptor with ab313731 at 1/50 (10.0 ug/ml) . Membranous staining on stormal cells in human liver. The section was incubated with ab313731 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PTPN12 with ab323839 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human breast cancer.
The primary antibody was incubated overnight at 4°C (shown in magenta). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling PTPN12 with ab323838 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human breast cancer.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PTPN12 with ab323838 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human colon.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab324337 at 1/50 (10.0 ug/ml) dilution.

Positive staining on human stomach.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PADI2 / PAD2 with ab324331 at 1/100 (5.0 ug/ml) dilution.

Positive staining on human colon.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on blood vessels of human placenta (PMID : 19625659) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EBI3 with ab314592 at 1/50 (10.0 ug/ml). Cytoplasmic staining on human placental trophoblasts. The section was incubated with ab314592 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD42b with ab313595 at 1/50 (10.0 ug/ml). Positive staining on platelets in human spleen.The section was incubated with ab313595 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cerebral cortex (PMID : 24114376) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of formalin fixed paraffin embedded Human spleen tissue labelling eIF4A3 with ab180573 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin.

Positive staining on human spleen.
The section was incubated with ab180573 at 4°C overnight.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with ab256494 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (ab214880). Positive staining on Human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling CD42b with ab313595 at 1/50 (10.0 ug/ml). Positive staining on megakaryocytes in human bone marrow.The section was incubated with ab313595 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Wilms Tumor Protein with ab314587 at 1/100 (5.0 ug/ml). Nuclear staining on human ovarian cancer. The section was incubated with ab314587 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SMARCA2 / BRM with ab307771 at 1/100 dilution (5.0 µg/ml). Nuclear staining on mouse cerebrum. The section was incubated with ab307768 at 4°C overnight (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Wilms Tumor Protein with ab314587 at 1/100 (5.0 ug/ml). Nuclear staining on mouse testis. The section was incubated with ab314587 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Wilms Tumor Protein with ab314587 at 1/100 (5.0 ug/ml). Nuclear staining on rat testis. The section was incubated with ab314587 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with ab256555 at 4℃ overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SMARCA2 / BRM with ab307770 at 1/100 dilution (5.0 µg/ml). Nuclear staining on mouse cerebrum. The section was incubated with ab307768 at 4°C overnight (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling alpha 1 Fetoprotein with ab307861 at 1/50 dilution (10.0 ug/ml). Negative control : no staining on rat liver. The section was incubated with ab307861 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Positive staining on rat testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded rat E16.5 liver tissue labeling alpha 1 Fetoprotein with ab307861 at 1/50 dilution (10.0 ug/ml). Positive staining on rat E16.5 liver. The section was incubated with ab307861 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SCP3 with ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on mouse testis. The section was incubated with ab314313 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling Sodium/Hydrogen Exchanger 1/NHE-1 with ab324336 at 1/50 (10.0 ug/ml) dilution.

Positive staining on mouse stomach.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (AB93684)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling Calcium Pump PMCA2 ATPase with ab324300 at 1/100 (5.0 ug/ml) dilution.

Positive staining on rat cerebrum.
The primary antibody was incubated overnight at 4°C (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Counterstained with DAPI.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)