Antigen Retrieval缓冲液(100X Citrate缓冲液)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on human tonsil The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Immunohistochemistry analysis of paraffin-embedded human cerebellum tissue sections labelling GFAP with ab4648 at 1/500 dilution (1 mg/mL). Sections were stained with ab4648 using the HRP/DAB staining. Sections were counterstained with hematoxylin/eosin. Antigen retrieval was heat mediated using Antigen retrieval buffer (100X citrate buffer) (pH 6.0) (ab93678) for 15 minutes. To the right is a region of cerebellar molecular layer containing the prominent cytoskeletal fibers of Bergmann glia which are strongly positive for GFAP. The middle shows a region of the granular layer and to the left is white matter, both of which contain GFAP positive astrocytes.The immunostaining was performed with the Vector ImmPress rat adsorbed horse anti-mouse IgG detection kit.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Immunohistochemical analysis of paraffin-embedded (A) FaDu (human pharyngeal carcinoma epithelial cell) cell pellet, (B) A431 (human epidermoid carcinoma epithelial cell) cell pellet and (C) K-562 (human chronic myelogenous leukemia lymphoblast) cell pellet labeling CELSR1 with ab322043 at 1/50 (10.36 μg/ml) dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on (A) FaDu cell pellet and (B) A431 cell pellet, no staining on (C) K-562 cell pellet.
The section was incubated with ab322043 at 4°C overnight.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval was performed with ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on mouse liver. The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on rat liver. The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Fibrillarin with ab314506 at 1/2000 (0.249 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Nucleolus staining on mouse testis. The section was incubated with ab314506 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (ab214880). Heat mediated antigen retrieval was performed using ab93678 (citrate buffer, pH 6.0)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

ab93678 Antigen Retrieval Bufer 100X Citrate Buffer

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PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Nuclear OPN expression in explanted hearts but not endomyocardial biopsies (EMBs)In Patient #1, nuclear OPN (shown in white) in ACTA1-positive cardiomyocytes (shown in red) is visible in failing native and transplanted hearts, but in none of the three serial follow-up EMB samples collected at 1 week, 1 month, and 1 year post first transplant. Representative confocal z-stack images are shown. Scale bar = 20 μm.

Image from Irion et al., Front Physiol., 11:928; doi: 10.3389/fphys.2020.00928. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/

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PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Citrate Buffer) (AB93678)

Immunohistochemical staining of GFP in the indicated genotypes (n=5 mice per group) : Immunohistochemical staining of GFP in the indicated genotypes (n=5 mice per group) : The paraffin-embedded sections were deparaffinized with xylene and alcohol and subjected to antigen retrieval by citrate buffer (Abcam, Cat# ab93678), Tris-EDTA buffer (Abcam, Cat# ab93684), EDTA buffer (Abcam, Cat# ab93680), or Tris buffer (Abcam, Cat# ab93682) in a 100°C cooker for 30 min. After blocking, the sections were incubated with primary antibodies at 4°C overnight. 1 : 500, Chicken anti-GFP, Abcam, Cat#ab13970. the mouse tissue sections were developed with an IHC Detection kit (Thermo Fisher, Cat#3600) according to the manufacturer's instructions.

Image from Wang et al., Nat Commun., 11(1):220. doi: 10.1038/s41467-019-14046-9. Reproduced under the Creative Commons license https://creativecommons.org/licenses/by/4.0/