AB7481

Normal山羊Serum

Name: Normal 山羊Serum (ab7481)| Abcam中文官网
Brand: Abcam Limited
SKU: AB7481
Rating: 5 (3 reviews)

Normal Goat Serum

(3 Reviews)

(482 Publications)

Normal Goat Serum/NGS (ab7481) is used for blocking in antibody-based applications e.g. IHC, ICC/IF or as an antibody diluent. It is effective in reducing nonspecific binding thereby maximizing signal-to-noise ratio.

- Alternative to bovine serum albumin (BSA) and non-fat dry milk
- Compatible with our Lab essentials imaging workflow offering
- Available in different sizes to fit your experimental needs
- Proven performance: cited in over 480 publications

2 Images

Immunohistochemistry (Frozen sections) - Normal Goat Serum (AB7481)

IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Normal Goat Serum (AB7481)

Slides were pretreated with Hydrogen Peroxide Block followed by 15% normal goat serum (Abcam, Cambridge, UK) for 1 hour. The primary antibody, anti-PTX3, N-terminal antibody produced in rabbit was diluted 1 : 300 in PBS with 2.5% goat serum (ab7481), applied to slides and incubated for 3 hours at 4°C. The primary antibody was omitted in the negative controls. The PTX3 binding was revealed using a universal secondary antibody polymer formulation conjugated to horseradish peroxidase (HRP). The HRP activity was subsequently visualized with diaminobenzidine (DAB) substrate/chromogen and counterstaining with hematoxylin was performed.

Witasp, Anna et al., PloS one vol. 8,5 e63493., Fig 3, doi:10.1371/journal.pone.0063493

Immunocytochemistry/ Immunofluorescence - Normal Goat Serum (AB7481)

ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Normal Goat Serum (AB7481)

Cells grown in 8-well chamber slides to 70% confluence were fixed with 4% paraformaldehyde (15 min at room temperature (RT)), and permeabilized with 100% ice cold methanol (10 min at 20°C). The slides were incubated with 10% normal goat serum (ab7481) in PBS for 1 hr, and incubated with anti-maspin (1 : 100) antibody alone or in a combination with either anti-lamin B (1 : 50), anti-HDAC1 (1 : 50) or anti-GRP78 (1 : 50) at 4°C overnight. Cells were washed and incubated for 2 hrs at room temperature (RT) with Alexa Fluor 488 (1 : 500) alone or in combination with Alexa Fluor 594 (1 : 500). The nuclei were counterstained with DAPI.
DU145 cells infected with adenovirus expressing either maspinWT or maspinD346E Confocal immunofluorescence imaging of maspin (green) and nuclear envelope marker lamin B (red) in DU145 cells after infection.

Dzinic, Sijana H et al., PloS one vol. 8,11 e74502., Fig 2, doi:10.1371/journal.pone.0074502

关键信息

应用

BL, IHC-Fr, IHC-P, ICC/IF

applications

形式

Liquid

form

存储溶液

Constituents: Whole serum

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "BL": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" } } }

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产品详情

Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.

The goat serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.

Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.

Serum can be used directly for blocking, or as a constituent of a blocking buffer.

Strain: Mixed breed and sex.

Raised in: Goat

Purity: Whole antiserum

This product is for research use only and not intended for diagnostic or therapeutic use of any kind.

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性能和储存信息

运输条件

Dry Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

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文献 (482)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:5384 PubMed40562742

2025

Polyglutamylation of microtubules drives neuronal remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Antoneta Gavoci,Anxhela Zhiti,Michaela Rusková,Maria M Magiera,Mengzhe Wang,Karin A Ziegler,Torben J Hausrat,Anselm I Ugwuja,Shreyangi Chakraborty,Stefan Engelhardt,Matthias Kneussel,Martin Balastik,Carsten Janke,Thomas Misgeld,Monika S Brill

Journal for immunotherapy of cancer 13: PubMed40541272

2025

HVJ-E links Apolipoprotein d to antitumor effects.

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Species

Unspecified reactive species

Airi Ishibashi,Noriko Ohta,Yuko Uegaki,Hidefumi Suzuki,Katsuya Fukino,Yuuta Hisatomi,Atsushi Tanemura,Riuko Ohashi,Koji Kitamura,Kotaro Saga,Yasuhide Yoshimura,Satoko Inubushi,Kyoso Ishida,Yoko Ino,Yayoi Kimura,Kenjiro Sawada,Tadashi Kimura,Eiji Kiyohara,Kosuke Yusa,Hidehisa Takahashi,Yasufumi Kaneda,Keisuke Nimura

Scientific reports 15:18734 PubMed40436929

2025

Systems serology-based comparison of humoral immune responses induced by liposome or aluminum hydroxide adjuvanted SARS-CoV-2 spike protein.

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Unspecified reactive species

Soo Ji Kim,Taewoo Kim,Satyanarayana Bejjani,Mi Sun Kim,Jung Hyuk Lee,Yuna Shin,Sun-Je Woo,Beom Min Cheon,Doyoung Kim,Sumin Lee,Eunjin Cho,Junhyeon Lee,Ruchirkumar Pansuriya,Wook-Jin Park,Gaurav Pandey,Ravi Ganapathy,Jung-Ah Choi,Ju Yeon Park,Deok Ryun Kim,Cheol-Heui Yun,Jae Seung Yang,Byoung-Shik Shim,Manki Song

Nature communications 16:4770 PubMed40404658

2025

Glioma-neuronal circuit remodeling induces regional immunosuppression.

Applications

Unspecified application

Species

Unspecified reactive species

Takahide Nejo,Saritha Krishna,Akane Yamamichi,Senthilnath Lakshmanachetty,Christian Jimenez,Kevin Y Lee,Donovan L Baker,Jacob S Young,Tiffany Chen,Su Su Sabai Phyu,Lan Phung,Marco Gallus,Gabriella C Maldonado,Kaori Okada,Hirokazu Ogino,Payal B Watchmaker,David Diebold,Abrar Choudhury,Andy G S Daniel,Cathryn R Cadwell,David R Raleigh,Shawn L Hervey-Jumper,Hideho Okada

Nature communications 16:4743 PubMed40399268

2025

Exercise-induced CLCF1 attenuates age-related muscle and bone decline in mice.

Applications

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Species

Unspecified reactive species

Jae Sook Kang,Jung Ha Kim,Min Ju Kim,Byungkuk Min,Seung-Min Lee,Ga-Yeon Go,Ji-Won Kim,Seongwan Kim,Ju Yeon Kwak,Sung-Wook Chun,Wook Song,Hyo Youl Moon,Sun Gun Chung,Dong-Ho Park,Ji Hoon Park,Chuna Kim,Kwang-Pyo Lee,Eun-Soo Kwon,Nacksung Kim,Ki-Sun Kwon,Yong Ryoul Yang

Journal, genetic engineering & biotechnology 23:100496 PubMed40390503

2025

Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells.

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Unspecified application

Species

Unspecified reactive species

Sudipta Deb Nath,Md Tamzid Hossain Tanim,Md Mahmudul Hasan Akash,Mohammad Golam Mostafa,Abu Ashfaqur Sajib

Cancer medicine 14:e70959 PubMed40387385

2025

FUT8 Is a Critical Driver of Prostate Tumour Growth and Can Be Targeted Using Fucosylation Inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla Bastian,Margarita Orozco-Moreno,Huw Thomas,Kirsty Hodgson,Eline A Visser,Emiel Rossing,Johan F A Pijnenborg,Nienke Eerden,Laura Wilson,Hasvini Saravannan,Oliver Hanley,Grace Grimsley,Fiona Frame,Ziqian Peng,Bridget Knight,Paul McCullagh,John McGrath,Malcolm Crundwell,Lorna Harries,Norman J Maitland,Rakesh Heer,Ning Wang,Ethan D Goddard-Borger,Ramon Hurtado Guerrero,Thomas J Boltje,Richard R Drake,Emma Scott,David J Elliott,Jennifer Munkley

BMC veterinary research 21:349 PubMed40380182

2025

Overexpression of MCL-1 in canine hepatocellular carcinoma and its efficacy as a prognostic marker.

Applications

Unspecified application

Species

Unspecified reactive species

Jehun Baek,Jaeho Cho,Hun-Kyeong Shin,Wan Hee Kim

Nature communications 16:4353 PubMed40348760

2025

Let-7 restrains an epigenetic circuit in AT2 cells to prevent fibrogenic intermediates in pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Seasock,Md Shafiquzzaman,Maria E Ruiz-Echartea,Rupa S Kanchi,Brandon T Tran,Lukas M Simon,Matthew D Meyer,Phillip A Erice,Shivani L Lotlikar,Stephanie C Wenlock,Scott A Ochsner,Anton Enright,Alex F Carisey,Freddy Romero,Ivan O Rosas,Katherine Y King,Neil J McKenna,Cristian Coarfa,Antony Rodriguez

Chemical biology & drug design 105:e70116 PubMed40346910

2025

Artesunate Enhances Sensitivity of Renal Cancer Cells to Sunitnib by Mediating Tripartite Motif Containing 24-Induced Ubiquitination of Paired Box 6.

Applications

Unspecified application

Species

Unspecified reactive species

Zelin Cui,Jianhua Wen,Guanglin Yang,Liwei Wei,Hao Chen,Qiyue Zhao,Shubo Yang,Jiayin Yu,Yichen Huang,Shuting Tan,Qizhou Mo,Min Qin,Jiwen Cheng

View all publications

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Normal山羊Serum

Normal Goat Serum

Immunohistochemistry (Frozen sections) - Normal Goat Serum (AB7481)

Immunocytochemistry/ Immunofluorescence - Normal Goat Serum (AB7481)