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Normal Bovine Calf Serum (sterile) (ab7479)

Submit a review Q&A (2)References (1)

概述

  • 产品名称

    Normal Bovine Calf Serum (sterile)

  • 宿主

    Cow

  • 经测试应用

    适用于: Blocking, IHC-P, IHC-Frmore details

  • 常规说明

    Total Protein Concentration : normal. Strain: Mixed breed and sex

    Raised in: Cow

    Purity: Whole antiserum

    Sterilization: This product was aseptically filtered through a 0.22 micron filter into clean, pre-sterilized containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7479于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
说明
Blocking
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab7479？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7479 被引用在 1 文献中.

  • Linghu C  et al. Spatial Multiplexing of Fluorescent Reporters for Imaging Signaling Network Dynamics. Cell 183:1682-1698.e24 (2020). PubMed: 33232692

客户评价及客户问答

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Question

What is the country of origin of ab138477 and ab7479?

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Answer

Both of these sera were producted using starting materials of North American origin in USDA inspected facilities.

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Question

I recently bought ab7479 (bovine serum albumin), I will be using it for blockage in western blotting for monoclonal and polyclonal antibodies for phosphorylated antigens. Since I was using BSA in a different (powder) form, I couldnt be sure of the dilution necessary for this product. There is no data on the protein amount in the datasheet. WB protocol that I follow recommends to use 5% weight to volume preparation for the powder form. What is the recommended amount for this product? Thank you very much.

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Answer

Thank you for your enquiry. The product ab7479 is not bovine serum albumin but bovine serum therefore it contains more proteins that just albumin. This product is typically used to reduce non-specific background staining which may be caused by a bovine secondary antibody during immunohistochemistry staining procedures. It may however work in western blotting. We typically recommend a 10% dilution (10ml serum plus 90ml PBST) as blocking agent for immunohistochemistry so I would recommend trying 5% and 10% (in TBST rather than PBST) and a one hour incubation. Please let me know if you need further help,

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