Z-VAD(OH)-FMK, Irreversible general caspase inhibitor

• IP

Lab

Immunoprecipitation - Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (AB120382)

MLKL (phospho S345) was immunoprecipitated from 1mg of L-929 (Mouse connective tissue fibroblast cells) whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic + 20 μM z-VAD compound (ab120382) for 8h using ab196436 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab196436 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : L-929 whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (ab120382) for 8h;10 μg (Input).
Lane 2 : ab196436 IP in L-929 whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (ab120382) for 8h.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab196436 in L-929 whole cell lysate treated with 20 ng/ml TNF alpha (ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (ab120382) for 8h.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-MLKL (phospho S345) antibody [EPR9515(2)] (<a href='/products/primary-antibodies/mlkl-phospho-s345-antibody-epr95152-ab196436'>ab196436</a>)

All lanes:

Immunoprecipitation - Recombinant human TNF alpha protein (<a href='/products/proteins-peptides/recombinant-human-tnf-alpha-protein-ab9642'>ab9642</a>)

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

• FuncS

PubMed

Functional Studies - Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (AB120382)

ZVAD-fmk treatment does not correct muscular dystrophy in Nol3-/-Sgcd-/- mice.

A : Muscle weights normalized to tibial length of quadriceps of mice treated with or without ZVAD-fmk for 4 weeks

B : Creatine Kinase levels

C : Quantification of the time to exhaustion as assessed by involuntary treadmill running measured from vehicle or ZVAD-fmk treated WT and Nol3-/- Sgcd-/- mice

D : Histologic images taken at 100x of Masson's trichrome stained sections of quadriceps from vehicle or ZVAD-fmk treated WT and Nol3^-/-Sgcd^-/- mice

Davis J et al. PLoS One. 2013; 8(12) : e82053. doi : 10.1371/journal.pone.0082053

Davis J et al. PLoS One. 2013; 8(12): e82053. doi: 10.1371/journal.pone.0082053 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Chemical Structure

Lab

Chemical Structure - Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (AB120382)

2D chemical structure image of ab120382, Z-VAD(OH)-FMK, Irreversible general caspase inhibitor