VU0155041 sodium salt，PAM for mGluR4 (ab120248)
Key features and details
- PAM for mGluR4; water soluble
- CAS Number: 1259372-69-4
- Purity: > 98%
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
VU0155041 sodium salt，PAM for mGluR4 -
描述
PAM for mGluR4; water soluble -
生物学描述
Positive allosteric selective modulator at mGluR4 (EC50 values are 798 and 693 nM at human and rat mGluR4 receptors, respectively). It has advantages over PHCCC (ab120043) as it is water soluble and more potent. Shows limited blood-brain barrier permeability. Active in behavioural models of Parkinson's disease following icv injection.
Also available in simple stock solutions (ab146686) - add 1 ml of water to get an exact, ready-to-use concentration. -
纯度
> 98% -
CAS编号
1259372-69-4 -
化学结构
性能
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化学名称
cis-2-(3,5-Dichlorophenylcarbamoyl)cyclohexanecarboxylic acid sodium salt -
分子量
338.16 -
分子式
C14H14Cl2NNaO3 -
PubChem识别号
52974247 -
存放说明
Store at Room Temperature. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM -
处理
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
[Na]OC(=O)C2CCCCC2C(=O)Nc1cc(Cl)cc(Cl)c1 -
来源
Synthetic
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研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120248于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - VU0155041 sodium salt, PAM for mGluR4 (ab120248)2D chemical structure image of ab120248, VU0155041 sodium salt, PAM for mGluR4
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Functional Studies - VU0155041 sodium salt, PAM for mGluR4 (ab120248)SKNSH cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of VU0155041 sodium salt (ab120248). Increased expression of Gli1 (ab49314) in SKNSH cells correlates with an decrease in VU0155041 sodium salt concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab49314 at 2.5 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti- rabbit antibody conjugated to HRP (ab97051) at 1/10000 and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (0)
ab120248 尚未被引用在任何文献中。