U0126，Selective MKK抑制剂(ab120241)
Key features and details
- Selective MKK inhibitor
- CAS Number: 109511-58-2
- Purity: > 98%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
U0126，Selective MKK抑制剂 -
描述
Selective MKK抑制剂 -
生物学描述
Selective non-competitive inhibitor of MAP kinase kinase (MKK). Inhibits MKK1, MKK2 (IC50 values of 0.07 and 0.06 μM, respectively) and MKK5 with little or no effect on a wide range of other kinases. Centrally active following systemic administration in vivo.
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纯度
> 98% -
CAS编号
109511-58-2 -
化学结构
性能
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化学名称
1,4-Diamino-2,3-dicyano-1,4-bis(2-aminophenylthio)butadiene -
分子量
380.49 -
分子式
C18H16N6S2 -
PubChem识别号
3006531 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
N/C(Sc1ccccc1N)=C(/C#N)C(C#N)=C(/N)Sc2ccccc2N -
来源
Synthetic
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研究领域
图片
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Chemical Structure - U0126, Selective MKK inhibitor (ab120241)2D chemical structure image of ab120241, U0126, Selective MKK inhibitor
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Western blot - U0126, Selective MKK inhibitor (ab120241)Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.
Lane 1: Marker
Lane 2: A431 lysate starved
Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)
Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution
Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.
Observed bands:
RSK1p90 (pS380) band size: 83 kDa
AKT1 (pS473) band size: 56 kDa
ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
RPS6 (pS235/236) band size: 31 kDa
Rab11a band size: 24 kDa
Unknown band: 130kDa
Performed under reducing conditions; Samples l
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (20)
ab120241 被引用在 20 文献中.
- van Krieken PP et al. Oncostatin M promotes lipolysis in white adipocytes. Adipocyte 11:315-324 (2022). PubMed: 35531859
- Sugita J et al. Cardiac macrophages prevent sudden death during heart stress. Nat Commun 12:1910 (2021). PubMed: 33771995
- van Krieken PP et al. Oncostatin M suppresses browning of white adipocytes via gp130-STAT3 signaling. Mol Metab 54:101341 (2021). PubMed: 34547509
- Lan CN et al. MAPK inhibitors protect against early-stage osteoarthritis by activating autophagy. Mol Med Rep 24:N/A (2021). PubMed: 34590154
- Luo Z et al. Metformin promotes survivin degradation through AMPK/PKA/GSK-3ß-axis in non-small cell lung cancer. J Cell Biochem N/A:N/A (2019). PubMed: 30793366