TMCB，Selective CK2抑制剂(ab120289)
Key features and details
- Selective CK2 inhibitor
- CAS Number: 905105-89-7
- Soluble in DMSO to 25 mM (with heating)
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
TMCB，Selective CK2抑制剂 -
描述
Selective CK2抑制剂 -
生物学描述
CK2 inhibitor (Ki = 0.25 μM), more selective than DMAT. Unlike DMAT, TMCB displays selectivity over PIM1, HIPK2 and DYRK1a (Ki values are 8.65, 15.25 and 11.90 μM, respectively) and a more favourable selectivity profile over a range of other kinases.
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CAS编号
905105-89-7 -
化学结构
性能
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化学名称
2-[4,5,6,7-Tetrabromo-2-(dimethylamino)-1H-benzo[d]imidazol-1-yl]acetic acid -
分子量
534.82 -
分子式
C11H9Br4N3O2 -
PubChem识别号
46943415 -
存放说明
Store at Room Temperature. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 25 mM (with heating) -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CN(C)c2nc1c(c(Br)c(Br)c(Br)c1Br)n2CC(=O)O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120289于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - TMCB, Selective CK2 inhibitor (ab120289)2D chemical structure image of ab120289, TMCB, Selective CK2 inhibitor
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Immunocytochemistry/ Immunofluorescence - TMCB, Selective CK2 inhibitor (ab120289)ab23423 staining cIAP2 in HeLa cells treated withTMCB (ab120289), by ICC/IF. Decrease in cIAP2 expression correlates with increased concentration of TMCB, as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120289 (TMCB) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab23423 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue. -
Functional Studies - TMCB, Selective CK2 inhibitor (ab120289)HeLa cells were incubated at 37°C for 24h with vehicle control (0 µM) and different concentrations of TMCB (ab120289). Decreased expression of cIAP2 in HeLa cells correlates with an increase TMCB concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 40 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab23423 at 2 µg/ml and ab1801 at 2 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (2)
ab120289 被引用在 2 文献中.
- Tsai CN et al. Serine 229 Balances the Hepatitis C Virus Nonstructural Protein NS5A between Hypo- and Hyperphosphorylated States. J Virol 93:N/A (2019). PubMed: 31511391
- Smith MC et al. CK2 inhibitors increase the sensitivity of HSV-1 to interferon-ß. Antiviral Res 91:259-66 (2011). PubMed: 21722672