Staurosporine,蛋白kinase抑制剂(ab120056)
Key features and details
- Protein kinase inhibitor
- CAS Number: 62996-74-1
- Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Staurosporine,蛋白kinase抑制剂 -
描述
蛋白kinase抑制剂 -
生物学描述
Potent, cell-permeable, broad spectrum inhibitor of protein kinases. Kinases inhibited include: protein kinase C (IC50 = 3 nM), cAMP-dependent protein kinase (IC50 = 8 nM) and p60v-src (IC50 = 6 nM).
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CAS编号
62996-74-1 -
化学结构
性能
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化学名称
[9S-(9α,10β,11β,13α)]-2,3,10,11,12,13-Hexahydro-10-methoxy-9-methyl-11-(methylamino)-9,13-epoxy-1H,9H-diindolo[1,2,3-gh:3,2,1-lm]pyrrolo[3,4-j][1,7]benzodiazonin-1-one -
分子量
466.54 -
分子式
C28H26N4O3 -
PubChem识别号
44259 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 50 mM -
处理
This product is supplied in one (or more) pack size which is freeze dried. Therefore the contents may not be readily visible, as they can coat the bottom or walls of the vial. Please see our FAQs and information page for more details on handling.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C6NCc5c2c4n(c1ccccc12)[C@@]3(C)O[C@H](C[C@@H](NC)[C@H]3OC)n7c4c(c56)c8ccccc78 -
来源
Synthetic
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研究领域
图片
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2D chemical structure image of ab120056, Staurosporine, Protein kinase inhibitor
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HeLa and Jurkat cells were treated with 1 µM Staurosporine (STS) (ab120056) for 4 hours in complete cell culture media to induce apoptosis and cleaved PARP protein. Untreated and STS treated HeLa and Jurkat lysates were prepared in 1X Cell Extraction Buffer PTR and tested the Cleaved PARP SimpleStepTM ELISA. Raw OD 450 nm values are shown for 500 µg/mL lysate loads.
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Lane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 µM staurpsorine (ab120056), 4 hours Load 20 µg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution
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Example of HeLa staurosporine (ab120056) treated cell lysate titration. Background-subtracted data values (mean +/- SD) are graphed.
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Example of IC50 determination. HeLa cells were treated with a dose titration of Staurosporine for 4 hours in complete media. Cells were cultured and treated in a 96-well cell culture microtiter plate. Lysates were prepared by direct in-well lysis without media removal: 2X Cell Extraction Buffer PTR was added to an equal volume of media and then resulting lysate was used directly in the Cleaved PARP SimpleStepTM ELISA assay. Raw values for triplicate measurements are plotted. The calculated IC50 is 0.77 µM.
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Demonstration of Cleaved PARP capture antibody specificity by western blot assay. 20 µg of HeLa extracts that were untreated or treated for 4 hours with 1 µM Staurosporine were analyzed by western blot. The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, Staurosporine treatment is required to detect cleaved PARP protein, as observed in the SimpleStep ELISA.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (27)
ab120056 被引用在 27 文献中.
- Miyakuni K et al. Genome-wide analysis of DNA methylation identifies the apoptosis-related gene UQCRH as a tumor suppressor in renal cancer. Mol Oncol 16:732-749 (2022). PubMed: 34133843
- Le TM et al. Ex vivo Induction of Apoptotic Mesenchymal Stem Cell by High Hydrostatic Pressure. Stem Cell Rev Rep 17:662-672 (2021). PubMed: 33128169
- Jabbari K et al. Protein Ligands in the Secretome of CD36+ Fibroblasts Induce Growth Suppression in a Subset of Breast Cancer Cell Lines. Cancers (Basel) 13:N/A (2021). PubMed: 34572749
- Wu N et al. Berberine ameliorates neuronal AD-like change via activating Pi3k/PGCε pathway. Biofactors 47:587-599 (2021). PubMed: 33740285
- Tomasek T et al. Cell-free hemoglobin-mediated human lung microvascular endothelial barrier dysfunction is not mediated by cell death. Biochem Biophys Res Commun 556:199-206 (2021). PubMed: 33848934