Src Kinase抑制剂I (SKI-1) (ab120839)
Key features and details
- Potent, selective and dual site Src tyrosine kinase family inhibitor
- CAS Number: 179248-59-0
- Purity: > 98%
- Soluble in DMSO to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Src Kinase抑制剂I (SKI-1) -
描述
Potent，selective and dual site Src tyrosine kinase family抑制剂 -
别名
- 4-(4-Phenoxyanilino)-6,7-dimethoxyquinazoline
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生物学描述
Potent, selective and dual site Src family tyrosine kinase inhibitor (IC50 values are 44 and 88 nM for Src and Lck, respectively). Cell-permeable, reversible and ATP-competitive. Interacts with both ATP and peptide-binding sites. Additionally inhibits VEGFR2 and C-fms (IC50 values are 0.32 and 30 µM, respectively). Induces apoptosis.
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纯度
> 98% -
CAS编号
179248-59-0 -
化学结构
性能
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化学名称
6,7-Dimethoxy-N-(4-phenoxyphenyl)-4-quinazolinamine -
分子量
373.41 -
分子式
C22H19N3O3 -
PubChem识别号
1474853 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 50 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
COc1cc4c(cc1OC)ncnc4Nc3ccc(Oc2ccccc2)cc3 -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120839于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Src Kinase Inhibitor I (SKI-1) (ab120839)2D chemical structure image of ab120839, Src Kinase Inhibitor I (SKI-1)
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Functional Studies - Src Kinase Inhibitor I (SKI-1) (ab120839)ab11512 staining E cadherin in SW480 cells treated with Src Kinase Inhibitor I (SKI-1) (ab120839), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of Src Kinase Inhibitor I (SKI-1), as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120839 (Src Kinase Inhibitor I (SKI-1)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (6)
ab120839 被引用在 6 文献中.
- Röselová P et al. Adhesion structures in leukemia cells and their regulation by Src family kinases. Cell Adh Migr 12:286-298 (2018). PubMed: 28678601
- Hock EM et al. Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Cell Rep 24:987-1000.e7 (2018). PubMed: 30044993
- Kumazoe M et al. Green Tea Polyphenol EGCG Upregulates Tollip Expression by Suppressing Elf-1 Expression. J Immunol 199:3261-3269 (2017). PubMed: 28954885
- Wang X et al. Human papillomavirus type 16 E6 oncoprotein promotes proliferation and invasion of non-small cell lung cancer cells through Toll-like receptor 3 signaling pathway. J Med Virol N/A:N/A (2017). Blocking ; Human . PubMed: 28480962
- McGee D et al. Cervical HSV-2 infection causes cervical remodeling and increases risk for ascending infection and preterm birth. PLoS One 12:e0188645 (2017). PubMed: 29190738
- Gomes JR et al. Transthyretin provides trophic support via megalin by promoting neurite outgrowth and neuroprotection in cerebral ischemia. Cell Death Differ 23:1749-1764 (2016). PubMed: 27518433