Spermidine hydrochloride，nNOS抑制剂(ab120057)
Key features and details
- Endogenous polyamine. nNOS inhibitor.
- CAS Number: 334-50-9
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Spermidine hydrochloride，nNOS抑制剂 -
描述
Endogenous polyamine. nNOS抑制剂. -
生物学描述
Endogenous polyamine, that binds to the polyamine modulatory site of the NMDA receptor. Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins.
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CAS编号
334-50-9 -
化学结构
性能
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化学名称
N-(3-Aminopropyl)butane-1,4-diamine trihydrochloride -
分子量
254.63 -
分子式
C7H19N3.3HCl -
PubChem识别号
9539 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NCCCNCCCCN.Cl.Cl.Cl -
来源
Synthetic
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研究领域
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Nitric oxide & oxidative stress
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
- Biochemicals
- Research Area
- Stroke
- Signaling
- Signal transduction
- Nitric oxide signaling
- NOS
- Inhibitors
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120057于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Spermidine hydrochloride, nNOS inhibitor (ab120057)2D chemical structure image of ab120057, Spermidine hydrochloride, nNOS inhibitor
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Functional Studies - Spermidine hydrochloride, nNOS inhibitor (ab120057)ab9969 staining IL10 in RAW 246.7 cells treated with spermidine hydrochloride (ab120057), by ICC/IF. Increase in IL10 expression correlates with increased concentration of spermidine hydrochloride, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120057 (spermidine hydrochloride) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab9969 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120057 被引用在 1 文献中.
- Ullrich M et al. Bio-orthogonal labeling as a tool to visualize and identify newly synthesized proteins in Caenorhabditis elegans. Nat Protoc 9:2237-55 (2014). PubMed: 25167056