Sodium butyrate，Histone deacetylase抑制剂(ab120948)
Key features and details
- Histone deacetylase inhibitor
- CAS Number: 156-54-7
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Sodium butyrate，Histone deacetylase抑制剂 -
描述
Histone deacetylase抑制剂 -
生物学描述
Histone deacetylase inhibitor (IC50 values are 0.3, 0.4 and 0.3 mM for HDAC1, 2 and 7, respectively). Shows no inhibition of HDAC6 and 10. Anticancer activity. Crosses the blood-brain barrier.
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CAS编号
156-54-7 -
化学结构
性能
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化学名称
Sodium butanoate -
分子量
110.09 -
分子式
C4H7NaO2 -
PubChem识别号
5222465 -
存放说明
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CCCC(=O)[O-].[Na+] -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120948于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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Western blot - Sodium butyrate, Histone deacetylase inhibitor (ab120948)
HeLa cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27) (ab4729) in HeLa cells correlates with an increase sodium butyrate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4729 at 1 µg/ml and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
HeLa cells were incubated at 37°C for 6h with vehicle control (0 µM) and different concentrations of sodium butyrate (ab120948). Increased expres
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Chemical Structure - Sodium butyrate, Histone deacetylase inhibitor (ab120948)2D chemical structure image of ab120948, Sodium butyrate, Histone deacetylase inhibitor -
Immunocytochemistry/ Immunofluorescence - Sodium butyrate, Histone deacetylase inhibitor (ab120948)ab4729 staining Histone H3 (acetyl K27) in HeLa cells. The cells were incubated with 10mM Sodium butyrate (ab120948) for 6 hours (Treated) or solvent-only for control purposes (Non-treated). Cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab4729 at 0.5µg/ml and ab7291 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a anti-rabbit AlexaFluor®488 secondary antibody (ab150077) at 2 μg/ml (shown in green) and a goat anti-mouse AlexaFluor®594 (ab150120) at 2 μg/ml (shown in pseudo colour red). Nuclear DNA was labelled in blue with DAPI. Negative controls: 1– Rabbit primary and anti-mouse secondary antibody; 2 – Mouse primary antibody and anti-rabbit secondary antibody. Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120948 被引用在 1 文献中.
- Bogacheva MS et al. Differences in definitive endoderm induction approaches using growth factors and small molecules. J Cell Physiol 233:3578-3589 (2018). PubMed: 29044512