Scriptaid，HDAC抑制剂(ab120883)
Key features and details
- HDAC inhibitor
- CAS Number: 287383-59-9
- Purity: > 98%
- Soluble in DMSO to 5 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Scriptaid，HDAC抑制剂 -
描述
HDAC抑制剂 -
生物学描述
Novel HDAC inhibitor, able to cause >100-fold increase in histone deacetylation. Less toxic than trichostatin A (ab120850). Induces cell cycle arrest in vitro and in vivo. -
纯度
> 98% -
CAS编号
287383-59-9 -
化学结构
性能
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化学名称
N-Hydroxy-1,3-dioxo-1H-benz[de]isoquinoline-2(3H)-hexanamide -
分子量
326.35 -
分子式
C18H18N2O4 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 5 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(C1=CC=CC2=C1C3=CC=C2)N(CCCCCC(NO)=O)C3=O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120883于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Scriptaid, HDAC inhibitor (ab120883)2D chemical structure image of ab120883, Scriptaid, HDAC inhibitor
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Immunocytochemistry/ Immunofluorescence - Scriptaid, HDAC inhibitor (ab120883)ab10812 staining histone H3 (acetyl K9) in A549 cells treated with scriptaid (ab120883), by ICC/IF. Increase in histone H3 (acetyl K9) expression correlates with increased concentration of scriptaid, as described in literature.
The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab120883 (scriptaid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab10812 (0.1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab120883 尚未被引用在任何文献中。