SB 203580 hydrochloride，p38 MAPK抑制剂(ab120235)
Key features and details
- p38 MAPK inhibitor; water soluble
- CAS Number: 869185-85-3
- Soluble in water to 25 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
SB 203580 hydrochloride，p38 MAPK抑制剂 -
描述
p38 MAPK抑制剂; water soluble -
生物学描述
Water soluble, cell-permeable p38 MAPK inhibitor. Shows selectivity over many other kinases but also shown to have some inhibitory activity against GAK, CK1 , RIP2 c-Raf and GSK3.
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CAS编号
869185-85-3 -
化学结构
性能
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化学名称
4-[4-(4-Fluorophenyl)-2-[4-(methylsulfinyl)phenyl]-1H-imidazol-5-yl]pyridine hydrochloride -
分子量
413.90 -
分子式
C21H16FN3OS.HCl -
PubChem识别号
16760644 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 25 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cl.CS(=O)c1ccc(cc1)c2nc(c(n2)c3ccncc3)c4ccc(F)cc4 -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120235于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - SB 203580 hydrochloride, p38 MAPK inhibitor (ab120235)2D chemical structure image of ab120235, SB 203580 hydrochloride, p38 MAPK inhibitor
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Western blot - SB 203580 hydrochloride, p38 MAPK inhibitor (ab120235)MDA-MB-231 cells cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor in MDA-MB-231 cells cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120235 被引用在 1 文献中.
- Gao R et al. R5 HIV-1 gp120 Activates p38 MAPK to Induce Rat Cardiomyocyte Injury by the CCR5 Coreceptor. Pathobiology 86:274-284 (2019). PubMed: 31574524