(S)-AMPA,激动剂(ab120005)
Key features and details
- AMPA agonist
- CAS Number: 83643-88-3
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
(S)-AMPA,激动剂 -
描述
AMPA激动剂 -
别名
- GLUR 1
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CAS编号
83643-88-3 -
化学结构
性能
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化学名称
(S)-α-Amino-3-hydroxy-5-methylisoxazole-4-propionic acid -
分子量
186.17 -
分子式
C7H10N2O4 -
PubChem识别号
158397 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
N[C@@H](Cc1c(C)onc1O)C(=O)O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120005于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - (S)-AMPA, agonist (ab120005)2D chemical structure image of ab120005, (S)-AMPA, agonist
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Functional Studies - (S)-AMPA, agonist (ab120005)Sims et al PLoS One. 2013;8(1):e53814. doi: 10.1371/journal.pone.0053814. Epub 2013 Jan 11. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Release of adenosine by depolarisation and agonists.
(Panel d) Adenosine released was evoked after depolarisation with AMPA.
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Functional Studies - (S)-AMPA, agonist (ab120005)ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (S)-AMPA (ab120005), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of (S)-AMPA, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120005 ((S)-AMPA) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (15)
ab120005 被引用在 15 文献中.
- Špirková A et al. Glutamate can act as a signaling molecule in mouse preimplantation embryos†. Biol Reprod 107:916-927 (2022). PubMed: 35746896
- Awasthi A et al. Synaptotagmin-3 drives AMPA receptor endocytosis, depression of synapse strength, and forgetting. Science 363:N/A (2019). PubMed: 30545844
- Sebe JY et al. Ca2+-Permeable AMPARs Mediate Glutamatergic Transmission and Excitotoxic Damage at the Hair Cell Ribbon Synapse. J Neurosci 37:6162-6175 (2017). PubMed: 28539424
- Winland CD et al. Inflammation alters AMPA-stimulated calcium responses in dorsal striatal D2 but not D1 spiny projection neurons. Eur J Neurosci 46:2519-2533 (2017). PubMed: 28921719
- Brandalise F & Gerber U Mossy fiber-evoked subthreshold responses induce timing-dependent plasticity at hippocampal CA3 recurrent synapses. Proc Natl Acad Sci U S A : (2014). PubMed: 24550458
