(S)-3,5-DHPG，group I mGlu激动剂(ab120007)
Key features and details
- Selective group I mGlu agonist
- CAS Number: 162870-29-3
- Soluble in water to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
(S)-3,5-DHPG，group I mGlu激动剂 -
描述
Selective group I mGlu激动剂 -
生物学描述
Selective group I mGlu agonist.
Also available in simple stock solutions (ab144484) - add 1 ml of water to get an exact, ready-to-use concentration. -
CAS编号
162870-29-3 -
化学结构
性能
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化学名称
(S)-3,5-Dihydroxyphenylglycine -
分子量
183.16 -
分子式
C8H9NO4 -
PubChem识别号
443586 -
存放说明
Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes. -
溶解度概述
Soluble in water to 50 mM -
处理
This compound is also unstable in alkaline solution when it rapidly forms a brown colouration it is therefore recommended that alkaline is not used to dissolve this compound.
We recommend stock solutions are made up in water, aliquoted immediately and stored at -20°C. They should be used within one week.Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Oc1cc(cc(O)c1)[C@H](N)C(=O)O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120007于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - (S)-3,5-DHPG, group I mGlu agonist (ab120007)2D chemical structure image of ab120007, (S)-3,5-DHPG, group I mGlu agonist
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Functional Studies - (S)-3,5-DHPG, group I mGlu agonist (ab120007)SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (S)-3,5-DHPG (ab120007). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase (S)-3,5-DHPG concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (30)
ab120007 被引用在 30 文献中.
- Ng AN et al. Amyloid-β1-42 oligomers enhance mGlu5R-dependent synaptic weakening via NMDAR activation and complement C5aR1 signaling. iScience 26:108412 (2023). PubMed: 38053635
- Špirková A et al. Glutamate can act as a signaling molecule in mouse preimplantation embryos†. Biol Reprod 107:916-927 (2022). PubMed: 35746896
- Serra I et al. Ca2+-modulated photoactivatable imaging reveals neuron-astrocyte glutamatergic circuitries within the nucleus accumbens. Nat Commun 13:5272 (2022). PubMed: 36071061
- Fernandes G et al. Correction of amygdalar dysfunction in a rat model of fragile X syndrome. Cell Rep 37:109805 (2021). PubMed: 34644573
- Redolfi N et al. A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals. Function (Oxf) 2:zqab012 (2021). PubMed: 35330679