(R,S)-CHPG，mGlu5激动剂(ab120039)
Key features and details
- Selective mGlu5 agonist
- CAS Number: 170846-74-9
- Soluble in 1 eq. NaOH to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
(R,S)-CHPG，mGlu5激动剂 -
描述
Selective mGlu5激动剂 -
生物学描述
Selective mGlu5 receptor agonist. Water soluble form available - see (ab120221)
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CAS编号
170846-74-9 -
化学结构
性能
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化学名称
(R,S)-2-Amino-2-(2-chloro-5-hydroxyphenyl)acetic acid -
分子量
201.61 -
分子式
C8H8NO3Cl -
PubChem识别号
3645780 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in 1 eq. NaOH to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
NC(c1cc(O)ccc1Cl)C(=O)O -
来源
Synthetic
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研究领域
相关产品
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120039于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - (R,S)-CHPG, mGlu5 agonist (ab120039)2D chemical structure image of ab120039, (R,S)-CHPG, mGlu5 agonist
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Functional Studies - (R,S)-CHPG, mGlu5 agonist (ab120039)SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of (R,S)-CHPG (ab120039). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase in (R,S)-CHPG concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32096 at 1/500 dilution and ab32515 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (3)
ab120039 被引用在 3 文献中.
- Fontanez-Nuin DE et al. Memory for fear extinction requires mGluR5-mediated activation of infralimbic neurons. Cereb Cortex 21:727-35 (2011). PubMed: 20705895
- Le Duigou C & Kullmann DM Group I mGluR agonist-evoked long-term potentiation in hippocampal oriens interneurons. J Neurosci 31:5777-81 (2011). PubMed: 21490219
- Besheer J et al. Interoceptive effects of alcohol require mGlu5 receptor activity in the nucleus accumbens. J Neurosci 29:9582-91 (2009). PubMed: 19641121