(R,S)-3,5-DHPG，group I mGlu receptor激动剂(ab120020)
Key features and details
- Group I mGlu receptor agonist
- CAS Number: 146255-66-5
- Purity: > 98%
Soluble in water to 10 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
(R,S)-3,5-DHPG，group I mGlu receptor激动剂 -
描述
Group I mGlu receptor激动剂 -
纯度
> 98% -
CAS编号
146255-66-5 -
化学结构
性能
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化学名称
2-amino-2-(3,5-dihydroxyphenyl)acetic acid
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分子量
183.16 -
分子式
C8H9NO4 -
PubChem识别号
108001 -
存放说明
Store at -20°C. Store In the Dark. Store under desiccating conditions. This product is air and light sensitive and impurities can occur as a result of air oxidation or due to metabolism by microbes. -
溶解度概述
Soluble in water to 10 mM
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处理
This compound is also unstable in alkaline solution when it rapidly forms a brown colouration it is therefore recommended that alkaline is not used to dissolve this compound.
We recommend stock solutions are made up in water, aliquoted immediately and stored at -20°C. They should be used within one week.Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=C(C=C(C=C1O)O)C(C(=O)O)N -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120020于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - (R,S)-3,5-DHPG, group I mGlu receptor agonist (ab120020)2D chemical structure image of ab120020, (R,S)-3,5-DHPG, group I mGlu receptor agonist
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Immunocytochemistry/ Immunofluorescence - (R,S)-3,5-DHPG, group I mGlu receptor agonist (ab120020)ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-3,5-DHPG (ab120020), by ICC/IF. Increase in FMRP expression correlates with increased concentration of (R,S)-3,5-DHPG, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120020 ((R,S)-3,5-DHPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (5)
ab120020 被引用在 5 文献中.
- Bowling H et al. Altered steady state and activity-dependent de novo protein expression in fragile X syndrome. Nat Commun 10:1710 (2019). PubMed: 30979884
- Lee K et al. Autism-associated Shank3 mutations alter mGluR expression and mGluR-dependent but not NMDA receptor-dependent long-term depression. Synapse 73:e22097 (2019). PubMed: 30868621
- Li JQ et al. Regulation of increased glutamatergic input to spinal dorsal horn neurons by mGluR5 in diabetic neuropathic pain. J Neurochem 112:162-72 (2010). PubMed: 19840219
- Roloff AM et al. Homer 1a gates the induction mechanism for endocannabinoid-mediated synaptic plasticity. J Neurosci 30:3072-81 (2010). PubMed: 20181604
- Musante V et al. Presynaptic mGlu1 and mGlu5 autoreceptors facilitate glutamate exocytosis from mouse cortical nerve endings. Neuropharmacology 55:474-82 (2008). PubMed: 18625255