PP 1，Src family tyrosine kinase抑制剂(ab120859)
Key features and details
- Potent Src family tyrosine kinase inhibitor
- CAS Number: 172889-26-8
- Purity: > 98%
- Soluble in DMSO to 25 mM and in ethanol to 10 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
PP 1，Src family tyrosine kinase抑制剂 -
描述
Potent Src family tyrosine kinase抑制剂 -
纯度
> 98% -
CAS编号
172889-26-8 -
化学结构
性能
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化学名称
1-(1,1-Dimethylethyl)-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine -
分子量
281.36 -
分子式
C16H19N5 -
PubChem识别号
1400 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 25 mM and in ethanol to 10 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cc1ccc(cc1)c3nn(c2ncnc(N)c23)C(C)(C)C -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120859于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - PP 1, Src family tyrosine kinase inhibitor (ab120859)2D chemical structure image of ab120859, PP 1, Src family tyrosine kinase inhibitor
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Functional Studies - PP 1, Src family tyrosine kinase inhibitor (ab120859)McGee et al PLoS One. 2017 Nov 30;12(11):e0188645. doi: 10.1371/journal.pone.0188645. eCollection 2017. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Herpes simplex virus-2 regulates ER-alpha and HA via Src kinase in ECT-1 cells.
(Panel B) ECT-1 cells were treated with vehicle (DMSO) or Src inhibitor (SKI/PP1). ER-alpha was quantified using densitometry following Western blot analysis (ANOVA with Tukey’s multiple comparison; NT vs VEH, n.s.; NT vs SKI/PPI, p< .02; VEH vs SKI/PPI, p< .01).
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Functional Studies - PP 1, Src family tyrosine kinase inhibitor (ab120859)ab11512 staining E cadherin in SW480 cells treated with PP1 (ab120859), by ICC/IF. Increase of E cadherin expression correlates with increased concentration of PP1, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120859 (PP1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab11512 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (1)
ab120859 被引用在 1 文献中.
- McGee D et al. Cervical HSV-2 infection causes cervical remodeling and increases risk for ascending infection and preterm birth. PLoS One 12:e0188645 (2017). PubMed: 29190738