(±)-Naringenin，Antioxidant (ab120958)
Key features and details
- Citrus flavonoid. Antioxidant, anti-inflammatory.
- CAS Number: 67604-48-2
- Purity: > 98%
- Soluble in DMSO to 100 mM and in ethanol to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
(±)-Naringenin，Antioxidant -
描述
Citrus flavonoid. Antioxidant，anti-inflammatory. -
纯度
> 98% -
CAS编号
67604-48-2 -
化学结构
性能
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化学名称
2,3-Dihydro-5,7-dihydroxy-2-(4-hydroxyphenyl)-4H-1-benzopyran-4-one -
分子量
272.26 -
分子式
C15H12O5 -
存放说明
Store at -20°C. It is important to note that this product is reported to be light sensitive. Store In the Dark. Store under desiccating conditions. -
溶解度概述
Soluble in DMSO to 100 mM and in ethanol to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120958于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - (±)-Naringenin, Antioxidant (ab120958)2D chemical structure image of ab120958, Naringenin, Antioxidant
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Functional Studies - (±)-Naringenin, Antioxidant (ab120958)Serum starved HepG2 cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of (±)-naringenin (ab120958). Increased expression of p38 (phospho T180 + Y182) (ab45381) in HepG2 cells correlates with an increase in (±)-naringenin concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab45381 at 1 µg /ml and ab8227 at 1/1000 dilution overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (0)
ab120958 尚未被引用在任何文献中。