(±)-Blebbistatin,抑制剂of myosin II (ab120425)
Key features and details
- Potent, selective, inhibitor of myosin II
- CAS Number: 674289-55-5
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
(±)-Blebbistatin,抑制剂of myosin II -
描述
Potent，selective,抑制剂of myosin II -
生物学描述
Cell-permeable, reversible inhibitor of non-muscle myosin II. Shows high affinity and selectivity for vertebrate and invertebrate Myosin II (IC50 values 0.5-5 μM). Inhibits actin-activated MgATPase activity and in vitro motility of class II myosins. Also inhibits contraction of the cytokinetic ring. Blocks cell blebbing and disrupts directed cell migration and cytokinesis.
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CAS编号
674289-55-5 -
化学结构
性能
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化学名称
(±)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-phenyl-4H-pyrrolo[2,3-b]quinolin-4-one -
分子量
292.34 -
分子式
C18H16N2O2 -
PubChem识别号
3476986 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cc2ccc3N=C1N(CCC1(O)C(=O)c3c2)c4ccccc4 -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120425于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - (±)-Blebbistatin, inhibitor of myosin II (ab120425)2D chemical structure image of ab120425, Blebbistatin, inhibitor of myosin II
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Functional Studies - (±)-Blebbistatin, inhibitor of myosin II (ab120425)ab32084 staining paxillin in MEF1 cells treated with (+/-)-blebbistatin (ab120425), by ICC/IF. Decreased membrane expression of paxillin correlates with increased concentration of (+/-)-blebbistatin, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120425 ((+/-)-blebbistatin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32084 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (34)
ab120425 被引用在 34 文献中.
- Sinha S et al. Dynamically Crosslinked Poly(ethylene-glycol) Hydrogels Reveal a Critical Role of Viscoelasticity in Modulating Glioblastoma Fates and Drug Responses in 3D. Adv Healthc Mater 12:e2202147 (2023). PubMed: 36239185
- Jiang C et al. Switch of cell migration modes orchestrated by changes of three-dimensional lamellipodium structure and intracellular diffusion. Nat Commun 14:5166 (2023). PubMed: 37620390
- Yu C et al. Chronological adhesive cardiac patch for synchronous mechanophysiological monitoring and electrocoupling therapy. Nat Commun 14:6226 (2023). PubMed: 37803005
- Hu S et al. Glycoprotein PTGDS promotes tumorigenesis of diffuse large B-cell lymphoma by MYH9-mediated regulation of Wnt-ß-catenin-STAT3 signaling. Cell Death Differ 29:642-656 (2022). PubMed: 34743203
- Lyu Y et al. Beat-to-beat dynamic regulation of intracellular pH in cardiomyocytes. iScience 25:103624 (2022). PubMed: 35005560
