NMDA，excitotoxic amino acid (ab120052)
Key features and details
- Excitotoxic amino acid
- CAS Number: 6384-92-5
- Soluble in water to 100 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
NMDA，excitotoxic amino acid -
描述
Excitotoxic amino acid -
生物学描述
Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy. Also available in Kit: Ionotropic agonists (ab120323).
Also available in simple stock solutions (ab146698) - add 1 ml of water to get an exact, ready-to-use concentration. -
CAS编号
6384-92-5 -
化学结构
性能
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化学名称
(R)-2-(Methylamino)succinic acid -
分子量
147.13 -
分子式
C5H9NO4 -
PubChem识别号
22880 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
OC(=O)C[C@@H](NC)C(=O)O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - NMDA, excitotoxic amino acid (ab120052)2D chemical structure image of ab120052, NMDA, excitotoxic amino acid
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Functional Studies - NMDA, excitotoxic amino acid (ab120052)Sims et al PLoS One. 2013;8(1):e53814. doi: 10.1371/journal.pone.0053814. Epub 2013 Jan 11. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Release of adenosine by depolarisation and agonists.
(Panel c) NMDA application also evoked neuronal depolarisation and firing accompanied by subsequent release of adenosine and inosine.
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Immunocytochemistry/ Immunofluorescence - NMDA, excitotoxic amino acid (ab120052)ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (18)
ab120052 被引用在 18 文献中.
- Xu D et al. Overexpressing NeuroD1 reprograms Müller cells into various types of retinal neurons. Neural Regen Res 18:1124-1131 (2023). PubMed: 36255002
- Špirková A et al. Glutamate can act as a signaling molecule in mouse preimplantation embryos†. Biol Reprod 107:916-927 (2022). PubMed: 35746896
- Bowers MS et al. NYX-2925 induces metabotropic N-methyl-d-aspartate receptor (NMDAR) signaling that enhances synaptic NMDAR and a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor. J Neurochem 152:523-541 (2020). PubMed: 31376158
- Kiragasi B et al. The auxiliary glutamate receptor subunit dSol-1 promotes presynaptic neurotransmitter release and homeostatic potentiation. Proc Natl Acad Sci U S A 117:25830-25839 (2020). PubMed: 32973097
- Girling KD et al. Activation of caspase-6 and cleavage of caspase-6 substrates is an early event in NMDA receptor-mediated excitotoxicity. J Neurosci Res 96:391-406 (2018). PubMed: 29193273