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AB146262

Nitrotetrazolium blue chloride (NBT), Chromogenic substrate

Nitrotetrazolium blue chloride (NBT), Chromogenic substrate

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(7 Publications)

MW 817.64 g/mol. Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
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Chemical Structure - Nitrotetrazolium blue chloride (NBT), Chromogenic substrate (AB146262)
  • Chemical Structure

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Chemical Structure - Nitrotetrazolium blue chloride (NBT), Chromogenic substrate (AB146262)

2D chemical structure image of ab146262, Nitrotetrazolium blue chloride (NBT), Chromogenic substrate

关键信息

CAS 号

298-83-9

形式

Solid

form

分子量

817.64 g/mol

分子式

C<sub>4</sub><sub>0</sub>H<sub>3</sub><sub>0</sub>Cl<sub>2</sub>N<sub>1</sub><sub>0</sub>O<sub>6</sub>

属性

Synthetic

溶解度

Soluble in water to 10 mM

生物学描述

Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

IUPAC 名

3,3'-[3,3'-Dimethoxy(1,1'-biphenyl)-4,4'-diyl]bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium dichloride

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搭配使用产品

Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

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Cell Lines & Lysates

AB110338

Bovine Heart Mitochondria

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  • 0
Reagents, Controls & Accessories

AB126587

10X Blocking Buffer

Western blot - 10X Blocking Buffer (AB126587)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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性能和储存信息

运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Store under desiccating conditions|The product can be stored for up to 12 months

Need more advice on solubility, usage and handling? Please visit our our product support page for more details.

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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

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Refer to SDS for further information.

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产品实验方案

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 17:336-364 PubMed39748059

2025

PlexinD1 is a driver and a therapeutic target in advanced prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wei,Jing Wang,Wen Guan,Jingjing Li,Tianjie Pu,Eva Corey,Tzu-Ping Lin,Allen C Gao,Boyang Jason Wu

Life (Basel, Switzerland) 14: PubMed39337855

2024

Investigating the Effects of Chelidonic Acid on Oxidative Stress-Induced Premature Cellular Senescence in Human Skin Fibroblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Burcu Turkoglu,Banu Mansuroglu

Scientific reports 13:19552 PubMed37945711

2023

STAT3 phosphorylation at serine 727 activates specific genetic programs and promotes clear cell renal cell carcinoma (ccRCC) aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

J Arévalo,I Campoy,M Durán,S Nemours,A Areny,M Vall-Palomar,C Martínez,G Cantero-Recasens,A Meseguer

Frontiers in pharmacology 13:1000278 PubMed36267290

2022

NQO1 protects against clioquinol toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jamuna Chhetri,Jem Dilek,Noel Davies,Glenn Jacobson,Robert Dallmann,Nuri Gueven

Neurobiology of disease 169:105737 PubMed35452786

2022

SOD1 mediates lysosome-to-mitochondria communication and its dysregulation by amyloid-β oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Norambuena,Xuehan Sun,Horst Wallrabe,Ruofan Cao,Naidi Sun,Evelyn Pardo,Nutan Shivange,Dora Bigler Wang,Lisa A Post,Heather A Ferris,Song Hu,Ammasi Periasamy,George S Bloom

Scientific reports 12:2354 PubMed35149730

2022

Relationship between oxidative stress and lifespan in Daphnia pulex.

Applications

Unspecified application

Species

Unspecified reactive species

Benedicth Ukhueduan,Charles Schumpert,Eunsuk Kim,Jeffry L Dudycha,Rekha C Patel

The Journal of biological chemistry 291:18016-29 PubMed27358405

2016

Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES.

Applications

Unspecified application

Species

Unspecified reactive species

Gaurab Sircar,Kuladip Jana,Angira Dasgupta,Sudipto Saha,Swati Gupta Bhattacharya
View all publications
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