Myricetin，irreversible TrxR抑制剂(ab120721)
Key features and details
- Potent antineoplastic, antioxidant and anti-inflammatory agent
- CAS Number: 529-44-2
- Purity: > 97%
- Soluble in DMSO to 100 mM and in ethanol to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
-
产品名称
Myricetin，irreversible TrxR抑制剂 -
描述
Potent antineoplastic，antioxidant and anti-inflammatory agent -
生物学描述
Directly inhibits MEK, JAK1, Akt and MKK4 kinase activity. Able to inhibit cytochrome P450 3A4 and 2C9 (IC50 = 7.81 and 13.5 μM, respectively).
-
纯度
> 97% -
CAS编号
529-44-2 -
化学结构
性能
-
化学名称
3,5,7-Trihydroxy-2-(3,4,5-trihydroxyphenyl)-4H-1-benzopyran-4-one -
分子量
318.24 -
分子式
C15H10O8 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM and in ethanol to 50 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
-
来源
Synthetic
-
研究领域
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Nitric oxide & oxidative stress
- Antioxidants
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Nitric oxide signaling
- Antioxidants
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- MAPK
- MKK/MEK
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Nitric oxide signaling
- Antioxidants
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Nitric oxide signaling
- Antioxidants
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120721于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
Functional Studies
Use at an assay dependent concentration. |
图片
-
Chemical Structure - Myricetin, irreversible TrxR inhibitor (ab120721)2D chemical structure image of ab120721, Myricetin, irreversible TrxR inhibitor
-
Functional Studies - Myricetin, irreversible TrxR inhibitor (ab120721)PC 12 cells were incubated at 37°C for 30 minutes with vehicle control (0 µM) and different concentrations of myricetin (ab120721). Increased expression of CaMKII (phospho T286) (ab32678) in PC 12 cells correlates with an increase in myricetin concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32678at 1/500 dilution and ab52476 at 1/500 dilution overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (0)
ab120721 尚未被引用在任何文献中。