Metformin hydrochloride，AMPK活化剂(ab120847)
Key features and details
- AMPK activator
- CAS Number: 1115-70-4
- Purity: > 99%
Soluble in water to 100 mM and in DMSO to 50 mM.
Sonication and heating in a water bath to approximately 45 °C are required to produce a clear solution in DMSO.
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
Metformin hydrochloride，AMPK活化剂 -
描述
AMPK活化剂 -
生物学描述
AMPK activator. Antidiabetic and anticancer activity in vivo. Rapidly crosses the blood-brain barrier.
Also available in simple stock solutions (ab146725) - add 1 ml of water to get an exact, ready-to-use concentration. -
纯度
> 99% -
CAS编号
1115-70-4 -
化学结构
性能
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化学名称
N,N-Dimethylimidodicarbonimidic diamide hydrochloride
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分子量
165.63 -
分子式
C4H11N5.HCl -
PubChem识别号
14219 -
存放说明
Store at Room Temperature. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 100 mM and in DMSO to 50 mM.
Sonication and heating in a water bath to approximately 45 °C are required to produce a clear solution in DMSO.
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处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CN(C)C(=N)N=C(N)N.Cl -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120847于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Metformin hydrochloride, AMPK activator (ab120847)2D chemical structure image of ab120847, Metformin hydrochloride, AMPK activator
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Immunocytochemistry/ Immunofluorescence - Metformin hydrochloride, AMPK activator (ab120847)ab1 staining HIF1α in MCF7 cells treated with metformin hydrochloride (ab120847), by ICC/IF. Decrease in HIF1α expression correlates with increased concentration of metformin hydrochloride, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (11)
ab120847 被引用在 11 文献中.
- Yang AJT et al. Influence of metabolic stress and metformin on synaptic protein profile in SH-SY5Y-derived neurons. Physiol Rep 11:N/A (2023). PubMed: 38010200
- Rai D et al. Evaluation of photoreceptor-directed fibroblasts derived from retinitis pigmentosa patients with defects in the EYS gene: a possible cost-effective cellular model for mechanism-oriented drug. Stem Cell Res Ther 13:157 (2022). PubMed: 35410372
- Farfan-Morales CN et al. The antiviral effect of metformin on zika and dengue virus infection. Sci Rep 11:8743 (2021). PubMed: 33888740
- Ruparelia AA et al. Metformin rescues muscle function in BAG3 myofibrillar myopathy models. Autophagy 17:2494-2510 (2021). PubMed: 33030392
- Kundu A et al. 14-3-3 proteins protect AMPK-phosphorylated ten-eleven translocation-2 (TET2) from PP2A-mediated dephosphorylation. J Biol Chem 295:1754-1766 (2020). PubMed: 31901078