LY 294002，PI3-kinase抑制剂(ab120243)
Key features and details
- PI3-kinase inhibitor
- CAS Number: 154447-36-6
- Purity: > 99%
- Soluble in DMSO to 25 mM and in ethanol to 5 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
LY 294002，PI3-kinase抑制剂 -
描述
PI3-kinase抑制剂 -
生物学描述
A highly selective inhibitor of phosphatidylinositol 3-kinase (IC50 = 1.4 μM). Selective over a range of other kinases including protein kinase C, protein kinase A, MAPK and PI4- kinase (IC50 > 50 μM).
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纯度
> 99% -
CAS编号
154447-36-6 -
化学结构
性能
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化学名称
2-(4-Morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one -
分子量
307.35 -
分子式
C19H17NO3 -
PubChem识别号
3973 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 25 mM and in ethanol to 5 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C2C=C(Oc1c(cccc12)c3ccccc3)N4CCOCC4 -
来源
Synthetic
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研究领域
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- PI3K/Akt signaling
- PI3-kinase
- Inhibitors
图片
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Chemical Structure - LY 294002, PI3-kinase inhibitor (ab120243)2D chemical structure image of ab120243, LY 294002, PI3-kinase inhibitor
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Western blot - LY 294002, PI3-kinase inhibitor (ab120243)Performed under reducing conditions; Samples loaded at 30 µg/lane. Membrane blocking and secondary antibody incubation performed in 5% milk/PBST. Primary antibody incubation performed in 5% BSA/PBST.
Lane 1: Marker
Lane 2: A431 lysate starved
Lane 3: A431 lysate starved treated with 100 ng/mL of EGF for 30 min
Lane 4: A431 lysate starved and pretreated for 2 hours with 10µM U0126 (ab120241)
Lane 5: A431 lysate starved and pretreated for 2 hours with 30µM Ly294002 (ab120243)
Primary: AKT/MAPK signaling pathway antibody cocktail at 1/250 dilution
Secondary: HRP conjugated Goat anti-Rabbit secondary antibody at 1:10,000 dilution.
Observed bands:
RSK1p90 (pS380) band size: 83 kDa
AKT1 (pS473) band size: 56 kDa
ERK1 (pY204) and ERK2 (pY187) band size: 43 kDa
RPS6 (pS235/236) band size: 31 kDa
Rab11a band size: 24 kDa
Unknown band: 130kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (41)
ab120243 被引用在 41 文献中.
- Choi YA et al. IRF3 Activation in Mast Cells Promotes FcεRI-Mediated Allergic Inflammation. Cells 12:N/A (2023). PubMed: 37296614
- Sang SQ et al. TRIM31 promotes the progression of oral squamous cell carcinoma through upregulating AKT phosphorylation and subsequent cellular glycolysis. Neoplasma 70:402-415 (2023). PubMed: 37498068
- Kong F et al. Inhibition of apoptosis-regulatory protein Siva-1 reverses multidrug resistance in gastric cancer by targeting PCBP1. Oncol Res 30:277-288 (2022). PubMed: 37303491
- Jiang T et al. Application of Bone Marrow Mesenchymal Stem Cells Effectively Eliminates Endotoxemia to Protect Rat from Acute Liver Failure Induced by Thioacetamide. Tissue Eng Regen Med 19:403-415 (2022). PubMed: 35122584
- Liu S et al. Protection of Human Lens Epithelial Cells from Oxidative Stress Damage and Cell Apoptosis by KGF-2 through the Akt/Nrf2/HO-1 Pathway. Oxid Med Cell Longev 2022:6933812 (2022). PubMed: 35222803
