Leptomycin B (ethanol solution)，Antibiotic，antifungal.抑制剂of CRM-1 mediated核export. (ab120501)
Key features and details
- Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
- CAS Number: 87081-35-4
- Supplied in ethanol (25 μg/ml)
- Form / State: Liquid
- Source: Steptomyces sp.
概述
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产品名称
Leptomycin B (ethanol solution)，Antibiotic，antifungal.抑制剂of CRM-1 mediated核export. -
描述
Antibiotic，antifungal.抑制剂of CRM-1 mediated核export. -
生物学描述
Potent inhibitor of CRM-1-mediated (exportin-1) nuclear export. Displays antibiotic, antifungal, and antitumor properties and blocks the cell cycle at G1 and G2 phases. Potent inhibitor of nucleo-cytoplasmic translocation of Rev. Also shown to suppress HIV-1 replication in human monocytes (IC50 = 600 pM).
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常规说明
Supplied in ethanol (25 µg/ml).
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CAS编号
87081-35-4 -
化学结构
性能
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化学名称
(2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-[(2S,3S)-3,6-Dihydro-3-methyl-6-oxo-2H-pyran-2-yl]-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18-nonadecapentaenoic acid -
分子量
540.74 -
分子式
C33H48O6 -
PubChem识别号
6917907 -
存放说明
Store at -20°C (desiccating conditions). -
溶解度概述
Supplied in ethanol (25 μg/ml) -
处理
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
O=C(O)C=C(/C)C[C@H](C)[C@@H](O)[C@H](C)C(=O)[C@H](C)C=C(/C)C=CC[C@@H](C)/C=C(C=C[C@@H]1OC(=O)C=C[C@@H]1C)CC -
来源
Steptomyces sp.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120501于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)2D chemical structure image of ab120501, Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
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Functional Studies - Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)ab38335 staining p75 in HeLa cells treated with Leptomycin B (ethanol solution) (ab120501), by ICC/IF. Increase in p75 expression in the nucleous and cytoplasm correlates with increased concentration of Leptomycin B (ethanol solution), as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120501 (Leptomycin B (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38335 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (2)
ab120501 被引用在 2 文献中.
- John SP et al. IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages. Cell Rep 25:95-106.e6 (2018). PubMed: 30282041
- Özdas S & Özdas T Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines. Turk J Biol 42:132-143 (2018). PubMed: 30814875