L-Glutamate，excitatory neurotransmitter (ab120049)
Key features and details
- Excitatory neurotransmitter
- CAS Number: 56-86-0
- Soluble in 1 eq. NaOH to 100 mM and in water to 25mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
L-Glutamate，excitatory neurotransmitter -
描述
Excitatory neurotransmitter -
生物学描述
Major excitatory neurotransmitter. Acts at glutamate receptors. Active in vivo.
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CAS编号
56-86-0 -
化学结构
性能
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化学名称
(S)-2-Aminopentanedioic acid -
分子量
147.13 -
分子式
C5H9NO4 -
PubChem识别号
33032 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in 1 eq. NaOH to 100 mM and in water to 25mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
OC(=O)CC[C@H](N)C(=O)O -
来源
Synthetic
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120049于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Functional Studies
Use at an assay dependent concentration. |
图片
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Chemical Structure - L-Glutamate, excitatory neurotransmitter (ab120049)2D chemical structure image of ab120049, L-Glutamate, excitatory neurotransmitter
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Immunocytochemistry/ Immunofluorescence - L-Glutamate, excitatory neurotransmitter (ab120049)ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with L-Glutamate (ab120049), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of L-Glutamate, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120049 (L-Glutamate) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (8)
ab120049 被引用在 8 文献中.
- Hu C et al. Different Aberrant Changes of mGluR5 and Its Downstream Signaling Pathways in the Scrapie-Infected Cell Line and the Brains of Scrapie-Infected Experimental Rodents. Front Cell Dev Biol 10:844378 (2022). PubMed: 35646890
- Wang C et al. The neuroprotective effect of melatonin in glutamate excitotoxicity of R28 cells and mouse retinal ganglion cells. Front Endocrinol (Lausanne) 13:986131 (2022). PubMed: 36313740
- Zhao X & Karpac J Glutamate metabolism directs energetic trade-offs to shape host-pathogen susceptibility in Drosophila. Cell Metab 33:2428-2444.e8 (2021). PubMed: 34710355
- Guo C et al. Purkinje cell outputs selectively inhibit a subset of unipolar brush cells in the input layer of the cerebellar cortex. Elife 10:N/A (2021). PubMed: 34369877
- Francescone R et al. Netrin G1 Promotes Pancreatic Tumorigenesis through Cancer-Associated Fibroblast-Driven Nutritional Support and Immunosuppression. Cancer Discov 11:446-479 (2021). PubMed: 33127842
- Mukherjee C et al. Oligodendrocytes Provide Antioxidant Defense Function for Neurons by Secreting Ferritin Heavy Chain. Cell Metab 32:259-272.e10 (2020). PubMed: 32531201
- Cheng K et al. Calsyntenin-1 Negatively Regulates ICAM5 Accumulation in Postsynaptic Membrane and Influences Dendritic Spine Maturation in a Mouse Model of Fragile X Syndrome. Front Neurosci 13:1098 (2019). PubMed: 31680833
- Pergner J & Kozmik Z Amphioxus photoreceptors - insights into the evolution of vertebrate opsins, vision and circadian rhythmicity. Int J Dev Biol 61:665-681 (2017). PubMed: 29319115