Forskolin,Adenylyl cyclase活化剂(ab120058)
Key features and details
- Adenylyl cyclase activator
- CAS Number: 66575-29-9
- Soluble in ethanol to 10 mM and in DMSO to 25 mM
- Form / State: Solid
- Source: Coleus forskohlii
概述
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产品名称
Forskolin,Adenylyl cyclase活化剂 -
描述
Adenylyl cyclase活化剂 -
生物学描述
Cell-permeable activator of adenylyl cyclase. Interacts directly with the catalytic subunit of the enzyme to increase intracellular cAMP levels. Positive inotropic, platelet antiaggregatory and antihypertensive actions in vivo. 3D Growth matrix component used in liver organoid generation.
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CAS编号
66575-29-9 -
化学结构
性能
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化学名称
(3R,4aR,5S,6S,6aS,10S,10aR,10bS)-Dodecahydro-6,10,10b-trihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-3-vinyl-1H-benzo[f]chromen-5-yl acetate -
分子量
410.50 -
分子式
C22H34O7 -
PubChem识别号
47936 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in ethanol to 10 mM and in DMSO to 25 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(=O)O[C@H]2[C@@H](O)[C@@H]1[C@](C)([C@@H](O)CCC1(C)C)[C@@]3(O)C(=O)C[C@](C)(C=C)O[C@]23C -
来源
Coleus forskohlii
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研究领域
- Biochemicals
- Pharmacology
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Biochemicals
- Research Area
- Heart disease
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Pain & inflammation
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Alzheimer's Disease
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Diabetes
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Hypertension
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Obesity
- Signaling
- Signal transduction
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
- Biochemicals
- Research Area
- Respiratory disease
- Signaling
- Inositol & cAMP signaling
- Adenylyl cyclase
- Activators
图片
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2D chemical structure image of ab120058, Forskolin, Adenylyl cyclase activator
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Effect of AC activation onto adult Mecp2X/Y and Mecp2308/Y Cortico-LA synapses.
A: A 10 minute application of the AC activator Forskolin (FSK, 50 µM) potentiates the Cortico-LA EPSC in young WT animals.
B: The same protocol is inefficient in adult WT mice (B1) and Mecp2308/Y mice (B2).
C: Summary plot of similar pharmacological experiments in WT and KO mice.
***: P<0.001.
Number of recorded cells is indicated.
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ab2541 staining XIAP in MCF7 cells treated with forskolin (ab120058), by ICC/IF. Increase in XIAP expression correlates with increased concentration of forskolin, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120058 (forskolin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab2541 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. -
1e7 3T3-L1 cells were treated for 15 minutes with 1 µM dexamethasone (ab120743) and 1 µg x mL-1 insulin (ab123768) with the addition of 50 µM forskolin (ab120058), 0.5 mM IBMX (ab120840) or 0.1 mM 8-CPT-cAMP (ab120424), extracted according to the protocol. Lysates were diluted 50-fold, and tested in duplicates (+/-SD).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (46)
ab120058 被引用在 46 文献中.
- Chen S et al. PAQR8 promotes breast cancer recurrence and confers resistance to multiple therapies. Breast Cancer Res 25:1 (2023). PubMed: 36597146
- Fernando SJA et al. Evidence that RXFP4 is located in enterochromaffin cells and can regulate production and release of serotonin. Biosci Rep 43:N/A (2023). PubMed: 36947541
- Xu X et al. Single-cell transcriptomic analysis uncovers the origin and intratumoral heterogeneity of parotid pleomorphic adenoma. Int J Oral Sci 15:38 (2023). PubMed: 37679344
- Sturiale V et al. Cell Cycle Reactivation, at the Start of Neurodegeneration, Induced by Forskolin and Aniline in Differentiated Neuroblastoma Cells. Int J Mol Sci 24:N/A (2023). PubMed: 37762676
- Machado H et al. Adipocyte lipolysis protects mice against Trypanosoma brucei infection. Nat Microbiol 8:2020-2032 (2023). PubMed: 37828246