FFN102 (Mini 102)，Fluorescent DAT and VMAT2底物(ab120866)
Key features and details
- Novel pH responsive FFN. Measures localization and activity of dopaminergic presynaptic terminals.
- CAS Number: 1234064-11-9
- Purity: > 98%
- Soluble in water to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
FFN102 (Mini 102)，Fluorescent DAT and VMAT2底物 -
描述
Novel pH responsive FFN. Measures localization and activity of dopaminergic presynaptic terminals. -
生物学描述
Novel, pH responsive fluorescent false neurotransmitter (FFN). Rodent DAT and VMAT2 substrate. Enables two-photon microscopic imaging of localization and activity of dopaminergic presynaptic terminals in the striatum of mouse acute brain slice. More selective for dopaminergic synapses than FFN511 (ab120331).
Exhibits greater fluorescence emission in neutral than acidic environments allowing optical measurement of synaptic vesicle content release.
Sufficiently bright, photostable and suitable for two-photon fluorescence microscopy and standard fluorescent microscopy. Compatible with GFP tags. -
纯度
> 98% -
CAS编号
1234064-11-9 -
化学结构
性能
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化学名称
4-(2-Aminoethyl)-6-chloro-7-hydroxy-2H-1-benzopyran-2-one 2,2,2-trifluoroacetate -
分子量
353.68 -
分子式
C11H10ClNO3.CF3CO2H -
PubChem识别号
91885430 -
存放说明
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 50 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
C1=C(C2=CC(=C(C=C2OC1=O)O)Cl)CCN.C(=O)(C(F)(F)F)O -
来源
Synthetic
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研究领域
图片
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Chemical Structure - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)2D chemical structure image of ab120866, FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate
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Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)All images provided by Thorsten Lau
Figure 1: Two neuronal cells stained with 50 μM FFN102 on differentiation day 10. Shown is a sum projection of a confocal z-stack. Accumulation of FFN102 can be observed along the neurites (arrows) and the cell soma (S).
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Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)Figure 2a: Images in the first row show a group of neuronal cells stained with 50 μM FFN102 (sum projection of a confocal stack). FFN102 localizes to structures on the cell soma (S) as well as neurites (arrows). Z indicates the area zoomed in for an additional z-stack.
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Fluorescent Cell Imaging - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)Figure 2b: Zoomed in area of “Z” from figure 2b. The arrow indicates a globular structure on a neurite. S indicates FFN102 positive structures on the cell soma. -
Cellular activation - FFN102 (Mini 102), Fluorescent DAT and VMAT2 substrate (ab120866)Figure 3: FFN102 dye uptake inhibition on addition of VMAT2 inhibitor RO4-1284
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (3)
ab120866 被引用在 3 文献中.
- Virdi GS et al. Protein aggregation and calcium dysregulation are hallmarks of familial Parkinson's disease in midbrain dopaminergic neurons. NPJ Parkinsons Dis 8:162 (2022). PubMed: 36424392
- Ho T et al. Vesicular expression and release of ATP from dopaminergic neurons of the mouse retina and midbrain. Front Cell Neurosci 9:389 (2015). PubMed: 26500494
- Rodriguez PC et al. Fluorescent dopamine tracer resolves individual dopaminergic synapses and their activity in the brain. Proc Natl Acad Sci U S A 110:870-5 (2013). PubMed: 23277566